Cloning and Sequencing of the Sox Genes in Chinemys reevesii

ZHANG Hai-Jun; NIE Liu-Wang; SHAN Xiang-Nian; ZHANG Xiao-Ai

Volume 22 Issue 4

Jul. 2001

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Article Navigation > Zoological Research > 2001 > 22(4): 336-339

ZHANG Hai-Jun, NIE Liu-Wang, SHAN Xiang-Nian, ZHANG Xiao-Ai. Cloning and Sequencing of the Sox Genes in Chinemys reevesii. Zoological Research, 2001, 22(4): 336-339.

Citation:

ZHANG Hai-Jun, NIE Liu-Wang, SHAN Xiang-Nian, ZHANG Xiao-Ai. Cloning and Sequencing of the Sox Genes in Chinemys reevesii. Zoological Research, 2001, 22(4): 336-339.

ZHANG Hai-Jun, NIE Liu-Wang, SHAN Xiang-Nian, ZHANG Xiao-Ai. Cloning and Sequencing of the Sox Genes in Chinemys reevesii. Zoological Research, 2001, 22(4): 336-339.

Citation:

ZHANG Hai-Jun, NIE Liu-Wang, SHAN Xiang-Nian, ZHANG Xiao-Ai. Cloning and Sequencing of the Sox Genes in Chinemys reevesii. Zoological Research, 2001, 22(4): 336-339.

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Cloning and Sequencing of the Sox Genes in Chinemys reevesii

1.
College of Life Science,Anhui Normal University,Wuhu 241000,China;2.Department of Biology,Medical College of Southeast University,Nanjing 210097,China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2001-08-22

Abstract

Abstract

Using a pair of degenerate primers based on the conservative region,HMG-box,of human SRY gene,four different fragments were amplified from both female and male Chinemys reevesii,then cloned by using pGEM-T vector and sequenced.The sequence result indicated their high homology to human Sox genes.The identities to human Sox genes in the DNA sequence and the amino acid sequence are 92%,91%,84%,92% and 100%,93%,98%,98%,respectively.It might be conclud ed that Sox gene was highly conservative in phylogenesis.Our work provides molecular data for the study of sex-determining mechanism of C.reevesii,which might be temperature-dependent sex determination.
- Chinemys reevesii,
- Sox gene,
- Cloning,
- Sequencing