Volume 28 Issue 2
Mar.  2007
Turn off MathJax
Article Contents
SHI Hai-jing, ZHU Zheng, LIU Long-ding, HU Yun-zhang, *. Morphological Properties of Mesenchymal Stem Cells Derived from Bone Marrow of Rhesus Monkeys (in English). Zoological Research, 2007, 28(2): 213-216.
Citation: SHI Hai-jing, ZHU Zheng, LIU Long-ding, HU Yun-zhang, *. Morphological Properties of Mesenchymal Stem Cells Derived from Bone Marrow of Rhesus Monkeys (in English). Zoological Research, 2007, 28(2): 213-216.

Morphological Properties of Mesenchymal Stem Cells Derived from Bone Marrow of Rhesus Monkeys (in English)

  • Received Date: 2006-11-06
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2007-04-22
  • To establish an in vitro system for isolating and culturing the mesenchymal stem cells (MSC) of Rhesus monkeys, and to provide research data for its further application, the bone marrow of Rhesus monkeys was collected and separated by gradient centrifugation to discard most of the blood cells. The MSC contained in the monocyte centrifuging layer was obtained and cultured in Dulbecco's modified media (low glucose, L-DMEM) supplemented with 10% Fetal bovine serum (FBS) and 1 ng/ml basic fibroblast growth factor (bFGF). The non-MSC was screened out by continuously renewing the medium. A passage culture was undertaken while the MSC monolayer formed. The spindle-shaped MSC formed a monolayer after 18 days of primary culturing, and the cells appeared in an oriented array with a swirling and irradiating growth trend. In the anaphase of passage culture, the cell proliferation rate was decreased and the morphology changed into triangular, polygon and flat appearance. These results suggested that mesenchymal stem cells (MSC) of the Rhesus monkey can be passaged in vitro with the established optimized culture system.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (3057) PDF downloads(2891) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return