Purification and Trypsin Inhibitory Activity of Xenopus laevis Serum Albumin

ZHANG Ying-xia; LEE Wen-hui; ZHANG Yun

Volume 26 Issue 6

Nov. 2005

Turn off MathJax

Article Contents

Article Navigation > Zoological Research > 2005 > 26(6): 622-626

ZHANG Ying-xia, LEE Wen-hui, ZHANG Yun. Purification and Trypsin Inhibitory Activity of Xenopus laevis Serum Albumin. Zoological Research, 2005, 26(6): 622-626.

Citation:

ZHANG Ying-xia, LEE Wen-hui, ZHANG Yun. Purification and Trypsin Inhibitory Activity of Xenopus laevis Serum Albumin. Zoological Research, 2005, 26(6): 622-626.

ZHANG Ying-xia, LEE Wen-hui, ZHANG Yun. Purification and Trypsin Inhibitory Activity of Xenopus laevis Serum Albumin. Zoological Research, 2005, 26(6): 622-626.

Citation:

ZHANG Ying-xia, LEE Wen-hui, ZHANG Yun. Purification and Trypsin Inhibitory Activity of Xenopus laevis Serum Albumin. Zoological Research, 2005, 26(6): 622-626.

PDF( 255 KB)

Purification and Trypsin Inhibitory Activity of Xenopus laevis Serum Albumin

1.
Department of Animal Toxinology, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2005-12-22

Abstract

Abstract

Frog albumin (XpA-serum) was purified from serum of Xenopus laevis by a combination of gel filtration and ion exchange chromatography steps. Like Bombina maxima albumin, XpA-serum exhibited trypsin inhibitory activity, which was lower than that of B. maxima albumin. XpA-serum at the concentration of 180 nM inhibited 84% activity of trypsin (30 nM). The equilibrium dissociation constants (KD) is 1.44×10-6 M as determined by Surface Plasmon Resonance. Western blot analysis revealed that XpA-serum was also distributed in the skin. It is deduced that serum albumin of amphibian possessing trypsin inhibitory activity can function directly or indirectly as a defensive substance against predators.
- Amphibian,
- Xenopus laevis,
- Serum albumin,
- Trypsin inhibitor