Purification and Characterization of Jerdonitin, a Non-hemorrhagic Metalloproteinase from Trimeresurus jerdonii Venom

CHEN Run-qiang; JIN Yang; WU Jian-bo; ZHONG Shu-rong; ZHU Shao-wen; LU Qiu-min et al.

Volume 26 Issue 6

Nov. 2005

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Article Navigation > Zoological Research > 2005 > 26(6): 616-621

CHEN Run-qiang, JIN Yang, WU Jian-bo, ZHONG Shu-rong, ZHU Shao-wen, LU Qiu-min et al.. Purification and Characterization of Jerdonitin, a Non-hemorrhagic Metalloproteinase from Trimeresurus jerdonii Venom. Zoological Research, 2005, 26(6): 616-621.

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Purification and Characterization of Jerdonitin, a Non-hemorrhagic Metalloproteinase from Trimeresurus jerdonii Venom

1.
Department of Animal Toxinology, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2005-12-22

Abstract

Abstract

Previously, we have purified Jerdonitin from Trimeresurus jerdonii venom. Compared with other P-Ⅱ class snake venom metalloproteinases (SVMPs), Jerdonitin has a primary structure comprising metalloproteinase and disintegrin domains. However, no hemorrhagic and fibrinogenolytic activities were detected for Jerdonitin. We thought that organic buffer of high performance liquid charamatography (HPLC) might affect its enzymatic activity. In this study, we purified Jerdonitin by another procedure excluding the HPLC. It was homogenous as judged by SDS-PAGE and had an apparent molecular weight of 36 kDa under non-reducing conditions and 38 kDa under reducing conditions, respectively. Like other typical metalloproteinases, Jerdonitin preferentially degraded alpha-chain of human fibrinogen and this fibrinogenolytic activity was completely inhibited by EDTA, but not by PMSF. It was interesting that Jerdonitin did not induce hemorrhage after intradermal injection in mice.
- Trimeresurus jerdonii,
- SVMP,
- Hemorrhage,
- Fibrinogenolytic activity