Study on Frozen Preservation and Resuscitation and Biological Characteristics on Primary Dispersed Tissue Cell of Rhesus Monkey's Kidney

ZHANG Mei-gui; DAI Yong-xiang; DONG De-xiang

Volume 4 Issue 1

Jan. 1983

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Article Navigation > Zoological Research > 1983 > 4(1): 95-101

ZHANG Mei-gui, DAI Yong-xiang, DONG De-xiang. Study on Frozen Preservation and Resuscitation and Biological Characteristics on Primary Dispersed Tissue Cell of Rhesus Monkey's Kidney. Zoological Research, 1983, 4(1): 95-101.

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Study on Frozen Preservation and Resuscitation and Biological Characteristics on Primary Dispersed Tissue Cell of Rhesus Monkey's Kidney

Institute of Medical Biology,Chinese Academy of Medical Sciences,Kunming

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 1983-02-22

Abstract

Abstract

Rhesus Monkey kidney trypsin-dispersed tissue cells were preserved at -196℃ and resuscitated.The biological characteristics of preserved cells were studied.The results of this study have indicated that cells frozen storage in -196 ℃ from 2-58 weeks,had a survival average rates of 63.3-74.4%.The reconstituted cells showed a good morphological appearance and,in general,required 6-7 days to form a dence monolayer,after rewarning at 37 ℃ for reculture.The cell monolayers were very susceptable to polioviruses.Karyotypes and chromosome analyses and sister chromatid exchange assay were unremarkable.Native dimethylsulfoxid (DMSO) may be used as a cryo-protective agent.