XU Qing-gang, CHEN Ke-ping, YAO Qin, LIU Xiao-yong. Screening and Cloning of RAPD Marker of Fluoride Tolerance Gene in Silkworm,Bombyx mori. Zoological Research, 2004, 25(1): 69-72.
Citation:
XU Qing-gang, CHEN Ke-ping, YAO Qin, LIU Xiao-yong. Screening and Cloning of RAPD Marker of Fluoride Tolerance Gene in Silkworm,Bombyx mori. Zoological Research, 2004, 25(1): 69-72.
XU Qing-gang, CHEN Ke-ping, YAO Qin, LIU Xiao-yong. Screening and Cloning of RAPD Marker of Fluoride Tolerance Gene in Silkworm,Bombyx mori. Zoological Research, 2004, 25(1): 69-72.
Citation:
XU Qing-gang, CHEN Ke-ping, YAO Qin, LIU Xiao-yong. Screening and Cloning of RAPD Marker of Fluoride Tolerance Gene in Silkworm,Bombyx mori. Zoological Research, 2004, 25(1): 69-72.
In this study,silkworm strain T6,tolerant to fluoride,and silkworm strain 733 xin,highly sensitive to fluoride,were used to construct the near-isogenic lines.300 random primers were used in RAPD amplification to DNAs of these lines.A molecular marker named S207 was found linked to the fluoride tolerance gene.Examination to F[2] segregated individuals of the above lines verified that this molecular marker was reliable.Subsequently,the molecular marker was cloned into a T vector (pUCm-T) for sequencing.Comparing with sequences available in the GenBank showed that this molecular marker was novel.We plan to convert it into a SCAR marker to facilitate establishment of a molecular marker assisted breeding system.
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