cDNA cloning and expression analysis of <i>MSTN</i> gene from <i>Schizopygopsis pylzovi</i>

CHAO Yan; ZHAO Lan-Ying; LI Chang-Zhong; XIE Bao-Sheng; SHEN Zhi-Xin; WANG Guo-Jie; WANG Zhen-Gang; LI Chao; BAI Bin-Qiang; ZHANG Hui; QI De-Lin

doi:10.3724/SP.J.1141.2012.05473

Volume 33 Issue 5

Sep. 2012

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Article Navigation > Zoological Research > 2012 > 33(5): 473-480

CHAO Yan, ZHAO Lan-Ying, LI Chang-Zhong, XIE Bao-Sheng, SHEN Zhi-Xin, WANG Guo-Jie, WANG Zhen-Gang, LI Chao, BAI Bin-Qiang, ZHANG Hui, QI De-Lin. cDNA cloning and expression analysis of MSTN gene from Schizopygopsis pylzovi. Zoological Research, 2012, 33(5): 473-480. doi: 10.3724/SP.J.1141.2012.05473

Citation:

CHAO Yan, ZHAO Lan-Ying, LI Chang-Zhong, XIE Bao-Sheng, SHEN Zhi-Xin, WANG Guo-Jie, WANG Zhen-Gang, LI Chao, BAI Bin-Qiang, ZHANG Hui, QI De-Lin. cDNA cloning and expression analysis of MSTN gene from Schizopygopsis pylzovi. Zoological Research, 2012, 33(5): 473-480. doi: 10.3724/SP.J.1141.2012.05473

Citation:

CHAO Yan, ZHAO Lan-Ying, LI Chang-Zhong, XIE Bao-Sheng, SHEN Zhi-Xin, WANG Guo-Jie, WANG Zhen-Gang, LI Chao, BAI Bin-Qiang, ZHANG Hui, QI De-Lin. cDNA cloning and expression analysis of MSTN gene from Schizopygopsis pylzovi. Zoological Research, 2012, 33(5): 473-480. doi: 10.3724/SP.J.1141.2012.05473

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cDNA cloning and expression analysis of MSTN gene from Schizopygopsis pylzovi

doi: 10.3724/SP.J.1141.2012.05473

1.
Animal Science Department of Agriculture and Animal Husbandry College, Qinghai University, Xining 810016, China;
2.
Departmentof Biological Sciences, Qinghai University, Xining 810016, China;
3.
Key Laboratory for Plateau Aquatic Organism and Environment of Qinghai Province, Fishery Environmental Monitoring Station of Qinghai Province, Xining 810012, China

Received Date: 2012-05-21
Rev Recd Date: 2012-09-04
Publish Date: 2012-10-10

Abstract

Abstract

Myostatin (MSTN) is a member of the TGF-β superfamily that acts as a negative regulator of skeletal muscle growth. A full-length, 2 180 bp, cDNA sequence of the myostatin gene from Schizopygopisis pylzovi was cloned with RT-PCR,5'-RACE and 3'-RACE and the cDNA clone included a 1 128 bp ORF, encoding a 375 amino acid peptide. Using PCR, we obtained the sequences of two introns of the MSTN gene and found that its structure in Schizopygopsis pylzovi was similar to that of other vertebrates, including three exons and two introns. Likewise, the putative MSTN peptide of Schizopygopsis pylzovi contains a conserved RXXR proteolytic cleavage domain, and 8 conserved cysteine residues in the C terminal of the protein, similar to other vertebrates. Phylogenetic analysis showed that the MSTN of Schizopygopsis pylzovi has high homology with other cyprinid fishes, but a low homology with mammals and birds. In the 9 examined tissues, the MSTN gene was highly expressed in heart, kidney, intestine and spermary, while weakly expressed in muscle, brain, fat, gill and hepatopancreas. Quantitative real-time PCR analysis showed that the expression of MSTN gene was different during embryo development, suggesting that the fish MSTN may not only play roles in muscle development but also contribute to other biological functions.
- Schizopygopsis pylzovi,
- Myostatin,
- Gene cloning,
- Expression

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References(24)

References

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