Volume 33 Issue 5
Sep.  2012
Turn off MathJax
Article Contents
CHAO Yan, ZHAO Lan-Ying, LI Chang-Zhong, XIE Bao-Sheng, SHEN Zhi-Xin, WANG Guo-Jie, WANG Zhen-Gang, LI Chao, BAI Bin-Qiang, ZHANG Hui, QI De-Lin. cDNA cloning and expression analysis of MSTN gene from Schizopygopsis pylzovi. Zoological Research, 2012, 33(5): 473-480. doi: 10.3724/SP.J.1141.2012.05473
Citation: CHAO Yan, ZHAO Lan-Ying, LI Chang-Zhong, XIE Bao-Sheng, SHEN Zhi-Xin, WANG Guo-Jie, WANG Zhen-Gang, LI Chao, BAI Bin-Qiang, ZHANG Hui, QI De-Lin. cDNA cloning and expression analysis of MSTN gene from Schizopygopsis pylzovi. Zoological Research, 2012, 33(5): 473-480. doi: 10.3724/SP.J.1141.2012.05473

cDNA cloning and expression analysis of MSTN gene from Schizopygopsis pylzovi

doi: 10.3724/SP.J.1141.2012.05473
  • Received Date: 2012-05-21
  • Rev Recd Date: 2012-09-04
  • Publish Date: 2012-10-10
  • Myostatin (MSTN) is a member of the TGF-β superfamily that acts as a negative regulator of skeletal muscle growth. A full-length, 2 180 bp, cDNA sequence of the myostatin gene from Schizopygopisis pylzovi was cloned with RT-PCR,5'-RACE and 3'-RACE and the cDNA clone included a 1 128 bp ORF, encoding a 375 amino acid peptide. Using PCR, we obtained the sequences of two introns of the MSTN gene and found that its structure in Schizopygopsis pylzovi was similar to that of other vertebrates, including three exons and two introns. Likewise, the putative MSTN peptide of Schizopygopsis pylzovi contains a conserved RXXR proteolytic cleavage domain, and 8 conserved cysteine residues in the C terminal of the protein, similar to other vertebrates. Phylogenetic analysis showed that the MSTN of Schizopygopsis pylzovi has high homology with other cyprinid fishes, but a low homology with mammals and birds. In the 9 examined tissues, the MSTN gene was highly expressed in heart, kidney, intestine and spermary, while weakly expressed in muscle, brain, fat, gill and hepatopancreas. Quantitative real-time PCR analysis showed that the expression of MSTN gene was different during embryo development, suggesting that the fish MSTN may not only play roles in muscle development but also contribute to other biological functions.
  • loading
  • [1]
    Acosta J, Carpio Y, Borreto I, González O, Estrada MP. 2005. Myostatin gene silenced by RNAi show a zebrafish giant phenotype [J]. J Biotechnol, 119(4): 324-331.
    [2]
    Amali AA, Lin CJF, Chen YH, Wang WL, Gong HY, Lee CY, Ko YL, Lu JK, Her GM, Chen TT, Wu JL. 2003. Up-regulation of muscle-specific transcription factors during embryonic somitogenesis of zebrafish (Danio rerio) by knock-down of myostatin [J]. Dev Dyn, 229(4): 847-856.
    [3]
    Burland TG. 2000. DNASTAR’s Lasergene sequence analysis software [J]. Methods Mol Biol, 132: 71–91.
    [4]
    Ji S, Losinski RL, Cornelius SG, Frank GR, Willis GM, Gerrard DE, Depreux FFS, Spurlock ME. 1998. Myostatin expression in porcine tissues: tissue specificity and developmental and postnatal [J]. Am J Physiol, 275(4 Pt 2): R1265-R1273.
    [5]
    Kocabas AM, Kucuktas H, Dunham RA, Liu ZJ. 2002. Molecular characterization and differential expression of the myostatin gene in channel catfish (Ictalurus punctatus) [J]. Biochim Biophys Acta, 1575(1-3): 99-107.
    [6]
    Kumar S, Tamura K, Nei M. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment [J]. Brief Bioinform, 5(2): 150-163.
    [7]
    Li XM, Fan W, Zhang B, Peng K, Wang YF, Hu W, Zhao HB. 2007. Cloning of myostatin of common carp and its expression pattern in different tissues [J]. Acta Hydrobiol Sin, 2007, 31(5): 643-648. [李兴美, 范巍, 张彬, 彭扣, 王玉凤, 胡炜, 赵浩斌. 2007. 鲤鱼肌肉生长抑制素基因(MSTN)的克隆及其组织表达特征. 水生生物学报, 2007, 31(5): 643-648.]
    [8]
    Lu XY, Zhang Y, Wang XG, Zhang YP, Xu JR, Lai R, Gu ZL. 2010. Identification and characterization of myostatin gene in rough-skinned sculp, trachidermus fasciatus [J]. Zool Res, 31(4): 387-394. [卢祥云, 张营, 王星果, 张燕萍, 徐建荣, 赖仭, 顾志良. 2010. 松江鲈肌肉生长抑制素基因克隆和序列特征分析. 动物学研究, 31(4): 387-394.]
    [9]
    McPherron AC, Lawler AM, Lee SJ. 1997. Regulation of skeletal muscle mass in mice by a new TGF-βsuperfamily member [J]. Nature, 387(6628): 83-90.
    [10]
    Patel K, Amthor H. 2005. The function of myostatin and strategies of myostatin blockade-new hope for therapies aimed at promoting growth of skeletal muscle [J]. Neuromuscul Disord, 15(2): 117-126.
    [11]
    Qi DL, Guo SC, Zhao XQ, Yang J, Tang WJ. 2007. Genetic diversity and historical population structure of Schizopygopsis pylzovi (Teleostei: Cyprinidae) in the Qinghai-Tibetan Plateau [J]. Freshw Biol, 52(6): 1090-1104.
    [12]
    Qi DL, Chao Y, Guo SC, Zhao XQ. 2008. Genetic structure of five Huanghe schizothoracin Schizopygopsis pylzovi populations based on mtDNA control region sequences [J]. Acta Zool Sin, 54(6): 972-980. [祁得林, 晁燕, 郭松长, 赵新全. 2008. 黄河裸裂尻鱼五种群mtDNA控制区的遗传结构. 动物学报, 54(6): 972-980.]
    [13]
    Qi DL, Li TP, Chao Y, Yang C. 2009. Taxonomic status of Schizopygopsis kessleri inferred from Cyt b gene sequence [J]. Chn J Zool, 44(4): 1-7. [祁得林, 李太平, 晁燕, 杨成. 2009. 利用Cyt b基因序列探讨柴达木裸裂尻鱼的分类学地位. 动物学杂志, 44(4): 1-7.]
    [14]
    Qi DL, Chao Y, Guo SC, Zhao LY, Li TP, Wei FL, Zhao XQ. 2012. Convergent, Parallel and Correlated Evolution of Trophic Morphologies in the Subfamily Schizothoracinae from the Qinghai- Tibetan Plateau [J]. PLoS ONE, 7(3): e34070.
    [15]
    Reisz-Porszasz S, Bhasin S, Artaza JN, Shen RQ, Sinha-Hikim I, Hogue A, Fielder TJ, Gonzalez-Cadavid NF. 2003. Lower skeletal muscle mass in male transgenic mice with muscle-specific overexpression of myostatin [J]. Am J Physiol Endocrinol Metab, 285(4): 876-888.
    [16]
    Rescan PY, Jutel I, Rallière C. 2001. Two myostatin genes are differentially expressed in myotomal muscles of the t rout (Oncorhynchus mykiss) [J]. J Exp Biol, 204(Pt 20): 3523-3529.
    [17]
    Rios R, Cameiro I, Arce VM, Devesa J. 2002. Myostatin is an inhibitor of myogenic differentiation [J]. Am J Physiol Cell Physiol, 282(5): C993-C999.
    [18]
    Saitoh K, Sado T, Mayden RL, Hanzawa N, Nakamura K, Nishida M, Miya M. 2006. Mitogenomic evolution and interrelationships of the Cypriniformes (Actinopterygii: Ostariophysi): the first evidence toward resolution of higher-level relationships of the world’s largest freshwater fish clade based on 59 whole mitogenome sequences [J]. J Mol Evol, 63(6): 826-841.
    [19]
    Sharma M, Kambadur R, Matthews KG, Somers WG, Devlin GP, Conaglen JV, Fowke PJ, Bass JJ . 1999. Myostatin, a transforming growth factor-beta superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct [J]. J Cell Physiol, 180(1): 1-9.
    [20]
    Vianello S, Brazzoduro L, Valle LD, Belvedere P, Colombo L. 2003. Myostatin expression during development and chronic stress in zebrafish (Danio rerio) [J]. J Endocrinol, 176(1): 47-59.
    [21]
    Wu YF. 1984. Systematics and taxonomy of the the Schizothoracine fishes in China [J]. Acta Biol Plat Sin, (3): 119-139. [武云飞. 1984. 中国裂腹鱼亚科鱼类分类与系统关系研究. 高原生物学集刊, (3): 119-139.]
    [22]
    Wu YF, Wu CZ. 1991. The Fishes of the Qinghai-Xizang Plateau [M]. Chengdu: Sichuan Publishing House of Science & Technology. [武云飞, 吴翠珍. 1991. 青藏高原鱼类. 成都: 四川科学技术出版社.]
    [23]
    Xu JY, Chen SL. 2008. Cloning and expression analysis of the myostatin (MSTN) gene in Paralicht hys olivaceus [J]. J Fisher Chn, 32(4): 497-506. [徐建勇, 陈松林. 2008. 牙鲆肌肉生长抑制素(MSTN)基因克隆. 水产学报, 32(4): 497-506.]
    [24]
    Yu JF, Zhang Y, Wang XG, Xu JR, Lu XY, Li JL, Gu ZL. 2010. Cloning and expression analysis of the myostatin gene in Elopichthys bambusa [J]. J Fisher Chn. 34(10): 1486-1493. [郁建锋, 张营, 王星果, 徐建荣, 卢祥云, 李建林, 顾志良. 2010. 鳡肌肉生长抑制素(MSTN)基因的克隆及其组织特异性表达分析. 水产学报, 34(10): 1486-1493.]
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (3203) PDF downloads(1912) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return