Digestive enzyme and alkaline phosphatase activities during the early stages of <em>Silurus soldatovi</em> development

LIU Wei; ZHANG Xiu-Mei; WANG Li-Bo

doi:10.3724/SP.J.1141.2010.06627

Volume 31 Issue 6

Nov. 2010

Turn off MathJax

Article Contents

Article Navigation > Zoological Research > 2010 > 31(6): 627-632

LIU Wei, ZHANG Xiu-Mei, WANG Li-Bo. Digestive enzyme and alkaline phosphatase activities during the early stages of Silurus soldatovi development. Zoological Research, 2010, 31(6): 627-632. doi: 10.3724/SP.J.1141.2010.06627

Citation:

LIU Wei, ZHANG Xiu-Mei, WANG Li-Bo. Digestive enzyme and alkaline phosphatase activities during the early stages of Silurus soldatovi development. Zoological Research, 2010, 31(6): 627-632. doi: 10.3724/SP.J.1141.2010.06627

Citation:

PDF( 241 KB)

Digestive enzyme and alkaline phosphatase activities during the early stages of Silurus soldatovi development

doi: 10.3724/SP.J.1141.2010.06627

1.
College of Fisheries, Ocean University of China, Qingdao 266003, China; 2. Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 3. The College of Animal Science and Technology, Northeast Agriculture University, Harbin 150030, China

Funds: Agricultural Science and Technology Fund of Department of Science and Technology (02EFN216900727)

More Information

Corresponding author: ZHANG Xiu-Mei
Received Date: 2010-02-24
Rev Recd Date: 2010-10-08
Publish Date: 2010-12-22

Abstract

Abstract

To provide a theoretical basis to improve the survival and growth rate and optimize diet of sheatfish (Silurus soldatovi), the activities of certain digestive enzymes and alkaline phosphatases were investigated during larval development ofone - ten day old individuals. Results indicated that sheatfish larva (~ three days after hatching) had high levels of alkaline protease activity, which peaked at five days old and dipped by eight days old, although the trend was generally upward. Acid protease activity at one - eight days old was low, after which it increased rapidly. Amylase activity reached the highest value at five days old, after which it began to decline. Lipase activity fluctuated markedly and showed two peaks at three - four days old and six - eight days old. Larval digestive enzyme activity and alkaline phosphatase activity were higher when fed live food than when fed an artificial diet. Throughout the early development process, alkaline protease activity was higher than acid protease, alkaline protease and amylase specific activity decreased significantly for eight-day-old transition larvae, while acid protease activity increased rapidly. These results indicate that the changes in digestive enzyme activity were relevant to digestive function conversion during fish larvae development. Alkaline phosphatase activity showed an upward trend over the first ten days of life, which indicated that the gastrointestinal function of sheatfish improved gradually.
- Silurus soldatovi,
- Larva,
- Juvenile,
- Digestive enzymes

FullText(HTML)

References(5)

References

[1]	Bong GK, Divakaran S, Brown CK, Os-Trowski AC, Divakaran S. 2001.Comparative digestive enzyme ontogeny in two marine larval fishes: Pacific threadfin, Polydactylus sexfilis and bluefin trevally, Caranx melampygus [J]. Fish Physiol Bioch, 24: 225-241.Cahu CL, Zambonino Infante JL. 1994. Early weaning of sea bass Dicentrarchus labrax larvae with a compound diet: Effect on digestive enzymes [J]. Comp Bioch Physiol, 109A: 213-222.Cahu CL, Zambonino Infante JL. 1998. Algal addition in sea bass (Dicentrarchus labrax) larvae rearing: Effect on digestive enzymes [J]. Aquaculture, 161(14): 479-489.Chen MY, Zhang XM. 2004. Recent advances in digestive physiology of marine fish larvae-juvenile [J]. Mar Fish Res, 25(3): 81-88. (in Chinese)Chen PJ, Wang ZG, Huang CN, Gu Y, Lu H. 1997. Variation of digestive enzyme activity in different development periods of Pagrosomus major [J]. J Ocean Taiwan Strait, 16(3): 245-248. Cousin JCB, Baudin-Laurencin F,Gabaudan J. 1987. Ontogeny of enzymatic activities in fed and fasting turbot Scophthalmus maximus L
[J]. Fish Biol, 30: 15-33.Cuvier-Peres, Kestemont. 2002. Development of some digestive enzymes in Eurasian perch larvae Perca fluviatilis [J]. Fish Physiol Bioch, 24: 279-285. Feng XY, Zheng JS, Wang ML. 2003. A study of the histochemistry on the digestive tract of the Sebastes schlegeli [J]. J Ocean Univ Qingdao, 33(3): 399-404. (in Chinese) Guan HH, Pan WZ, Chen J, Zhao CG, Liu W. 2006. Post-embryonic development of liver and pancreas and absorption of yolk in Silurus soldatovi, Silurus asotus and F1 [J] J Fish Sci Chn, 13(3): 460-464.(in Chinese)Kolkovski S. 2001. Digestive enzymes in fish larvae and juvenile-implications and applications to formulated diets [J]. Aquaculture, 200: 181-201Munilla-Moran R, Stark JR, Barbour A. 1990. The role of exogenous enzymes in digestion incultured turbot Scophthalmus maximus larvae
[J]. Aquaculture, 88: 337-350.Oozeki Y, Bailey KM. 1995. Ontogenetic development of digestive enzyme activities in larval walleye pollock Theragra chalcogramma [J]. Mar Biol, 122:177-186.Pan LQ, Wang KX. 1997. The experimental studies on activities of digestive enzyme in the larvae Penaeus chinensis [J].J Fish Chn, 21(1): 26-31. (in Chinese)
[4]	Pan WZ,Yin HB, Liu W, Lu TY, Ren B, Zhao CG. 2000.
[5]	[if supportFields]>