Volume 31 Issue 6
Nov.  2010
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CHANG Yu-Mei, GAO Guo-Qiang, XU Li-Hua, LIU Jin-Liang, LIU Chun-Lei, Liang Li-qun. Correlation and location of EST markers with cold tolerance trait of common carp (Cyprinus carpio L.). Zoological Research, 2010, 31(6): 581-586. doi: 10.3724/SP.J.1141.2010.06581
Citation: CHANG Yu-Mei, GAO Guo-Qiang, XU Li-Hua, LIU Jin-Liang, LIU Chun-Lei, Liang Li-qun. Correlation and location of EST markers with cold tolerance trait of common carp (Cyprinus carpio L.). Zoological Research, 2010, 31(6): 581-586. doi: 10.3724/SP.J.1141.2010.06581

Correlation and location of EST markers with cold tolerance trait of common carp (Cyprinus carpio L.)

doi: 10.3724/SP.J.1141.2010.06581
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  • Author Bio:

    CHANG Yu-Mei,email:ymchang@fishbreeding.org

  • Received Date: 2010-07-13
  • Rev Recd Date: 2010-10-20
  • Publish Date: 2010-12-22
  • Expressed sequence tags (ESTs) are parts of complementary DNAs (cDNAs) with certain gene function.It provides us more information than other neutral markers. The association of EST markers with phenotypes can increase our understanding of the biochemical pathways and mechanisms affecting economically important traits. In this study, 12 candidate EST markers isolated from the cold-induced brain cDNA of common carp (Cyprinus carpio L.) were conducted the correlation analysis of marker and cold tolerance trait of common carp using GLM model of SPSS 17.0 software firstly, then tried to locate them in the genetic linkage map using OneMap software. As a result, eight out of 12 candidate EST makers were separately located in six linkage groups, in which marker CC009(P<0.05) and CC115 (P<0.01) were associated with cold tolerance andmapped to LG38 and LG2, respectivey. Homology identity alignments showed that marker CC009 was highly homologous to the known Uridine-cytidine kinase I of Danio rerio with an identity of 94%, and marker CC115 was lowly homologous to the putative glycosyl transferase of Prochlorococcus marinus with an identity of 56%.
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  • [1]
    Atwood HL, Tomasso JR, Webb K,Gatlin DM.2003. Low-temperature tolerance of Nile tilapia, Oreochromis niloticus: Efforts of environmental and dietary factors [J]. Aquacult Res, 34: 241-251.Burton RF. 1996. The dependence of normal arterial blood pH on sodium concentration in teleost fish [J]. Comp Biochem Physiol, 114A(2): 111-116.Burton RF. 2002. Evolutionary determinants of normal arterial plasma pH in ectothermic vertebrates [J]. J Exp Biol, 205: 641-650.Cato SA, Gardner RC, Kent J, Richardson TE. 2001. A rapid PCR-based method for genetically mapping ESTs [J]. Theor Appl Genet, 102: 296-306.Chang YM, Kuang YY, Cao DC, Liang LQ, Sun XW, Lei QQ. 2006b. Effects of cooling temperature stress on hematology and serum chemistry values of Cyprinus carpio [J]. J Fish Sci Chn, 30(5): 701-706. [常玉梅, 匡友谊, 曹鼎臣, 梁利群, 孙效文, 雷清泉. 2006b. 低温胁迫对鲤血液学和血清生化指标的影响. 水产学报, 30(5): 701-706.]Chang YM, Liang LQ, Sun XW, Cao DC, Lei QQ. 2006a. The changes of serum pH values of common carp during cooling temperature
    [J]. J North Agrc Univ, 37(3): 357-36. [常玉梅, 梁利群, 孙效文, 曹鼎臣, 雷清泉. 2006a. 低温鲤鱼血清pH值的变化. 东北农业大学学报, 37(3): 357-36.]Dai HQ. Wang HX, Shen YM. 2007. Recent advances in antibiotic glycosyltransferases [J]. Chn J Antibiot, 32(5): 257-262. [代焕琴, 王浩鑫, 沈月毛. 2007. 抗生素糖基转移酶研究进展. 中国抗生素杂志, 32(5): 257-262.]Devires AL. 1971. Glycoproteins as biological antifreeze agents in Antarctic fishes [J]. Science, 172 (3988): 1152-1155.Diegane ND, Chen YY, Lin YH, Vaseeharan B, Chen JC. 2007. The immune response of tilapia Oreochromis mossambicus and its susceptibility to Streptococcus iniae under stress in low and high temperatures[J]. Fish Shellfish Immunol, 22(6):686-694.Gao GQ, Chang YM, Kuang YY, Liang LQ. 2010. The construction of genetic linkage map of common carp (Cyprinus carpio L.) using OneMap software [J]. J Fish Sci Chn, 34(5): 649-654.[高国强, 常玉梅, 匡友谊, 梁利群. 2010. 利用OneMap软件构建鲤遗传连锁图谱. 水产学报, 34(5): 649-654.]Hatey F, Tosser-Klopp G, Clouscard-martinato C, Mulsant P, Gasser F. 1998. Expressed sequenced tags for genes: A review [J]. Genet Select Evol, 30: 521-541.Ji X, Sun XW, Yang LG, Li YJ, Liu HJ. 2008.Paternity testing of mitogynogenetic Japanese flounder (Paralichthys olivaceus) using microsatellite loci [J]. Zool Res, 29(1): 25-30. [季旭, 孙效文, 杨立更, 李雅娟, 刘海金. 2008. 微卫星标记对牙鲆有丝分裂雌核发育家系的亲子鉴定. 动物学研究, 29(1): 25-30.]Jiang YQ, Chen XF, Liu PT, Zhang NC. 1990. Study on antifreeze protein in fishes II. The cloning of antifreeze protein gene cDNA of pseudopleuronectes yokohamae and its expression in E.coil [J]. J. Genet. Genomics, 17(3) 202-210. [蒋耀青,陈雄风, 刘澎涛, 张乃昌. 1990. 鱼类抗冻蛋白的研究II. 黄盖鲽抗冻蛋白基因cDNA 的克隆及其在大肠杆菌中的表达. 遗传学报, 17(3) 202-210.]Li SF, Li CH, Dey M, Gagalac F, Dunham R.2002. Cold tolerance of three strains of Nile tilapia, Oreochromis niloticus, in China [J]. Aquaculture, 213: 123-129.Li Y, Ding L, Li Y, Hou N, Chang YM, Liang LQ , Lei QQ. 2008. Influence on the expression quantity of five genes in the different tissues of common carps Cyprinus carpio under the low temperature[J]. Acta Zool Sin, 54(3): 460-466. [李勇, 丁雷, 李言, 侯宁, 常玉梅, 梁利群, 雷清泉. 2008. 低温对鲤鱼的5个基因在不同组织中表达量的影响. 动物学报, 54(3): 460-466.]Li Y, He W, Ding L, Li Y, Liang LQ, Lei QQ. 2009. Construction and identification of cDNA library from common carp(Cyprinus carpio Linnaeus) brain [J]. J Fish Sci Chn, 16(2): 147-155. [李言, 何薇, 丁雷, 李勇, 梁利群, 雷清泉. 2009. 鲤脑组织cDNA文库的构建及脑室管膜素基因鉴定. 中国水产科学, 16(2): 147-155.]Liang LQ, Chang YM, Zou QW, Lei QQ. 2009.Cloning and correlation analysis of cold adaptation of NADH—quinone oxidoreductase 3 subunit gene from common carp (Cyprinus carpio) [J]. J Jishou Univ: Nat Sci ed, 30(5): 77-81. [梁利群, 常玉梅, 邹庆薇, 雷清泉. 2009. 鲤NADH泛醌氧化还原酶亚基3基因的克隆及低温适应相关性分析. 吉首大学学报:自然科学版, 30(5): 77-81.]Liang LQ, Li SW, Chang YM, Gao JS, Sun XW, Lei QQ. 2006. Application of suppression subtractive hybridization (SSH) to study of cold tolerance in common carp (Cyprinus carpio) [J]. J Fish Sci Chn, 13(2): 193-199. [梁利群, 李绍戊, 常玉梅, 高俊生, 孙效文, 雷清泉. 2006. 抑制消减杂交技术在鲤鱼抗寒研究中的应用. 中国水产科学, 13(2): 193-199.]Liang LQ, Sun XW, Shen JB, Yan XC. 1997. RAPD analysis for the character of cold tolerance of the common carp [J]. J Fish Sci Chn, 4(3): 89-91. [梁利群, 孙效文, 沈俊宝, 闫学春. 1997. 鲤鱼抗寒性状的RAPD分析. 中国水产科学, 4(3): 89-91.]Liang LQ, Sun XW. 2003. Mapping cold tolerance strain on genetic linkage map of common carp [J]. J Dalian Fish Univ, 18(4): 278-281.
    [梁利群, 孙效文. 2003. 鲤耐寒性状分子标记在遗传连锁图上的定位. 大连水产学院学报, 18(4): 278-281.]Liu ZJ, Karsi A, Dunham RA. 1999. Development of polymorphic EST markers suitable for genetic linkage mapping of catfish[J]. Mar Biotechnol, 1: 437- 447.Naruse K, Tamka M, Mita K, Shima A, Postlethwait J, Mitani H. 2004. A medaka gene map: the trace of an cestral vertebrate proto chromosomes revealed by comparative gene mapping [J]. Genome Res, 14: 820-828.Pan X, Liang LQ, Lei QQ. 2008. Selection of microsatellite molecular markers associated with the character of cold tolerance of common carp[J]. J Harbin Inst Technol, 40(6): 915- 918. [潘贤, 梁利群, 雷清泉. 2008. 筛选与鲤鱼抗寒性状相关的微卫星标记. 哈尔滨工业大学学报, 40(6): 915- 918.]Perry GML, Martyniuk CM, Ferguson MM, DanzmannRG. 2005. Genetic parameters for upper thermal tolerance and growth-related traits in rainbow trout (Oncorhynchus mykiss)[J]. Aquaculture, 250(1-2): 120-128.Song W, Chen XY, Xu JR, Zhang ZY. 2003. Research progress in forest tree genetic linkage map construction and its future prospects
    [J]. Hereditas, 25(6): 749-756. [宋婉, 陈晓阳, 续九如, 张志毅. 2003. 林木遗传连锁图谱构建研究进展与发展方向. 遗传, 25(6): 749-756.]Wang CY, Yu XM, Tong JG. 2008. Development and characterization of polymorphic EST markers in common carp
    [J]. Acta Hydrobiol Sin, 32(2): 207-212.[王春艳, 俞小牧, 童金苟. 2008. 鲤鱼多态性EST标记的筛选与特性分析. 水生生物学报, 32(2): 207-212.]Wang JY, Zhu SG, Xu CF. 2002. Biochemistry: Next volume [M]. 3rd ed. Beijing: High Education Publishing House, 391-398. [王镜岩, 朱圣庚, 徐长法. 2002. 生物化学: 下册. 3版. 北京: 高等教育出版社, 391-398.]Woods I G, Kelly P D, Chu F, Ngo-Hazelett P, Yan YL, Huang H, Postlethwait JH, Talbot WS. 2000. A comparative map of the zebrafish genome [J]. Genom Res, 10: 1903-1914.Wu RL, Ma CX, Painter I, Zeng ZB. 2002. Simultaneous maximum likelihood estimation of linkage and linkage phases in outcrossing species
    [J]. Theor Pop Biol, 61: 349-363.Yamashita M, Ojima N, Sakamoto T. 1996. Induction of proteins in response to cold of rainbow trout cells [J]. FEBS Lett, 382: 261-264.Yang QX, Cao JW, Huang GJ. 1997. Glycosyltransferases and the enzymes of deglycosylation [J]. Amin Acids Bio tic Resou, 19(2): 38-43. [杨清香, 曹军卫, 黄国锦. 1997. 糖基转移酶和去糖基化酶. 氨基酸和生物资源, 19(2): 38-43.]Yu ZN, Guo XM. 2003. Genetic linkage map of the eastern oyster Crussostrea vgialca Gmelin[J]. Biol Bull, 204: 327-338.Zhang X, Wu HZ, Wei W, Xu HY, Zhang YX. 2009. Cloning, expression and characterization of antifreeze protein IV-like gene from large yellow croaker (Pseudosciaena crocea) [J]. Food Drug, 11(9): 15-19. [张晓, 吴海珍, 卫玮, 徐红艳, 张元兴. 2009. 大黄鱼类lV型抗冻蛋白基因的克隆、表达及功能研究. 食品与药品, 11(9): 15-19.]Zhu XP, Xia SL, Zhang Y, Liu JZ. 1997. Preliminary study on transgenic mudcarp of antifreeze protein gene [J]. J Fish Sci Chn, 4(2): 79-80.
    [朱新平, 夏仕玲, 张跃, 刘家照. 1997. 转抗冻蛋白基因鲮鱼的初步研究. 中国水产科学, 4(2): 79-80.]

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