Electron Microscopic Analysis of CAT Pore-orming Effect on Human Erythrocyte Membrane

LIU Shu-bai; HE Ying-ying; QIAN Jin-qiao; ZHANG Yun

doi:10.3724/SP.J.1141.2008.05503

Volume 29 Issue 5

Sep. 2008

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LIU Shu-bai, HE Ying-ying, QIAN Jin-qiao, ZHANG Yun. Electron Microscopic Analysis of CAT Pore-orming Effect on Human Erythrocyte Membrane. Zoological Research, 2008, 29(5): 503-510. doi: 10.3724/SP.J.1141.2008.05503

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LIU Shu-bai, HE Ying-ying, QIAN Jin-qiao, ZHANG Yun. Electron Microscopic Analysis of CAT Pore-orming Effect on Human Erythrocyte Membrane. Zoological Research, 2008, 29(5): 503-510. doi: 10.3724/SP.J.1141.2008.05503

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Electron Microscopic Analysis of CAT Pore-orming Effect on Human Erythrocyte Membrane

doi: 10.3724/SP.J.1141.2008.05503

1.
Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming Yunnan 650223, China; 2. Department of Anesthesiology, the First Affiliated Hospital of Kunming Medical College, Kunming Yunnan 650032, China;3. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China

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Corresponding author: ZHANG Yun
Received Date: 2008-04-22
Rev Recd Date: 1900-01-01
Publish Date: 2008-10-22

Abstract

Abstract

βγ-CAT is a naturally existing multifunctional protein complex of non-lens βγ-crystallin and trefoil factor from Bombina maxima skin secretions. In this paper, we first analysed the time-response curve of hemolysis and intracellular potassium efflux induced by βγ-CAT on human erythrocytes. After βγ-CAT (3 nmol/L) in human erythrocytes was treated at 37℃ for 5 min, we found that about 93.31±5.89% (P<0.01) of the intracellular potassium ions leaked, whereas the percentage of hemolysis was only13.12±1.92 % (P<0.05). We employed electron microscopy to observe the erythrocytes’ morphological changes and found erythrocytes were swollen and some had globular structures occurring on the cell surfaces, and fast releasing hemoglobins. These results indicated that the hemolytic effect of βγ-CAT on human erythrocyte membranes was due to intracellular potassium ion’s rapid efflux. These results demonstrate morphological proof in understanding the mechanism of βγ-CAT pore-forming effect on human erythrocytes.
- Trefoil factor,
- Non-lens &beta,
- &gamma,
- -crystallin,
- &beta,
- &gamma,
- -CAT,
- Pore-forming protein,
- Scanning electron microscopy,
- Transmission electron microscopy