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Ying Wang, Hua-Min Wang, Yan Zhou, Ling-Hong Hu, Jing-Ming Wan, Ji-Hui Yang, Hong-Bo Niu, Xiu-Ping Hong, Peng Hu, Liang-Biao Chen. Dusp1 regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity. Zoological Research, 2023, 44(1): 126-141. doi: 10.24272/j.issn.2095-8137.2022.397
Citation: Ying Wang, Hua-Min Wang, Yan Zhou, Ling-Hong Hu, Jing-Ming Wan, Ji-Hui Yang, Hong-Bo Niu, Xiu-Ping Hong, Peng Hu, Liang-Biao Chen. Dusp1 regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity. Zoological Research, 2023, 44(1): 126-141. doi: 10.24272/j.issn.2095-8137.2022.397

Dusp1 regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity

doi: 10.24272/j.issn.2095-8137.2022.397
All Illumina RNA sequencing data from this project were deposited at NCBI under BioProjectID PRJNA602594, National Genomics Data Center (GSA: CRA008815), and Science Data Bank (DOI: 10.57760/sciencedb.06296).
Supplementary data to this article can be found online.
The authors declare that they have no competing interests.
Y.W. and L.B.C. designed the experiment, completed data analysis, and wrote the manuscript. H.M.W., L.H.H., J.M.W., J.H.Y., H.B.N., and X.P.H. performed the experiments. Y.Z. and P.H. helped with analyses and constructive discussion. All authors read and approved the final version of the manuscript.
Funds:  This work was supported by the National Key Research and Development Program of China (2018YFD0900601) and National Natural Science Foundation of China (32130109)
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  • Corresponding author: E-mail: lbchen@shou.edu.cn
  • Received Date: 2022-10-26
  • Accepted Date: 2022-11-23
  • Published Online: 2022-11-23
  • Temperature tolerance restricts the distribution of a species. However, the molecular and cellular mechanisms that set the thermal tolerance limits of an organism are poorly understood. Here, we report on the function of dual-specificity phosphatase 1 (DUSP1) in thermal tolerance regulation. Notably, we found that dusp1-/- zebrafish grew normally but survived within a narrowed temperature range. The higher susceptibility of these mutant fish to both cold and heat challenges was attributed to accelerated cell death caused by aggravated mitochondrial dysfunction and over-production of reactive oxygen species in the gills. The DUSP1-MAPK-DRP1 axis was identified as a key pathway regulating these processes in both fish and human cells. These observations suggest that DUSP1 may play a role in maintaining mitochondrial integrity and redox homeostasis. We therefore propose that maintenance of cellular redox homeostasis may be a key mechanism for coping with cellular thermal stress and that the interplay between signaling pathways regulating redox homeostasis in the most thermosensitive tissue (i.e., gills) may play an important role in setting the thermal tolerance limit of zebrafish.
  • All Illumina RNA sequencing data from this project were deposited at NCBI under BioProjectID PRJNA602594, National Genomics Data Center (GSA: CRA008815), and Science Data Bank (DOI: 10.57760/sciencedb.06296).
    Supplementary data to this article can be found online.
    The authors declare that they have no competing interests.
    Y.W. and L.B.C. designed the experiment, completed data analysis, and wrote the manuscript. H.M.W., L.H.H., J.M.W., J.H.Y., H.B.N., and X.P.H. performed the experiments. Y.Z. and P.H. helped with analyses and constructive discussion. All authors read and approved the final version of the manuscript.
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