A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats

Ying Yuan; Wei-Yi Zhang; Bai-Gao Yang; Dong-Ke Zhou; Lu Xu; Yong-Meng He; Hao-Yuan Zhang; Cheng-Li Liu; Yue-Hui Ma; Ming-Xing Chu; Wen-Guang Zhang; Hui-Jiang Gao; Lin Jiang; Fu-Ping Zhao; Lu-Pei Zhang; Ri-Su Na; Baatarchogt Oyungerel; Yan-Guo Han; Yan Zeng; Shi-Zhi Wang; Huai-Zhi Jiang; Hong-Ping Zhang; Xun-Ping Jiang; Jian-Ning He; Hao Liang; Kaushalendra Kaushalendra; Ya-Wang Sun; Yong-Fu Huang; Yong-Ju Zhao; Zhong-Quan Zhao; Guang-Xin E

doi:10.24272/j.issn.2095-8137.2022.384

Volume 44 Issue 2

Mar. 2023

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Article Navigation > Zoological Research > 2023 > 44(2): 303-314

Ying Yuan, Wei-Yi Zhang, Bai-Gao Yang, Dong-Ke Zhou, Lu Xu, Yong-Meng He, Hao-Yuan Zhang, Cheng-Li Liu, Yue-Hui Ma, Ming-Xing Chu, Wen-Guang Zhang, Hui-Jiang Gao, Lin Jiang, Fu-Ping Zhao, Lu-Pei Zhang, Ri-Su Na, Baatarchogt Oyungerel, Yan-Guo Han, Yan Zeng, Shi-Zhi Wang, Huai-Zhi Jiang, Hong-Ping Zhang, Xun-Ping Jiang, Jian-Ning He, Hao Liang, Kaushalendra Kaushalendra, Ya-Wang Sun, Yong-Fu Huang, Yong-Ju Zhao, Zhong-Quan Zhao, Guang-Xin E. A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats. Zoological Research, 2023, 44(2): 303-314. doi: 10.24272/j.issn.2095-8137.2022.384

Citation:

Ying Yuan, Wei-Yi Zhang, Bai-Gao Yang, Dong-Ke Zhou, Lu Xu, Yong-Meng He, Hao-Yuan Zhang, Cheng-Li Liu, Yue-Hui Ma, Ming-Xing Chu, Wen-Guang Zhang, Hui-Jiang Gao, Lin Jiang, Fu-Ping Zhao, Lu-Pei Zhang, Ri-Su Na, Baatarchogt Oyungerel, Yan-Guo Han, Yan Zeng, Shi-Zhi Wang, Huai-Zhi Jiang, Hong-Ping Zhang, Xun-Ping Jiang, Jian-Ning He, Hao Liang, Kaushalendra Kaushalendra, Ya-Wang Sun, Yong-Fu Huang, Yong-Ju Zhao, Zhong-Quan Zhao, Guang-Xin E. A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats. Zoological Research, 2023, 44(2): 303-314. doi: 10.24272/j.issn.2095-8137.2022.384

Citation:

Ying Yuan, Wei-Yi Zhang, Bai-Gao Yang, Dong-Ke Zhou, Lu Xu, Yong-Meng He, Hao-Yuan Zhang, Cheng-Li Liu, Yue-Hui Ma, Ming-Xing Chu, Wen-Guang Zhang, Hui-Jiang Gao, Lin Jiang, Fu-Ping Zhao, Lu-Pei Zhang, Ri-Su Na, Baatarchogt Oyungerel, Yan-Guo Han, Yan Zeng, Shi-Zhi Wang, Huai-Zhi Jiang, Hong-Ping Zhang, Xun-Ping Jiang, Jian-Ning He, Hao Liang, Kaushalendra Kaushalendra, Ya-Wang Sun, Yong-Fu Huang, Yong-Ju Zhao, Zhong-Quan Zhao, Guang-Xin E. A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats. Zoological Research, 2023, 44(2): 303-314. doi: 10.24272/j.issn.2095-8137.2022.384

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A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats

doi: 10.24272/j.issn.2095-8137.2022.384

1.
College of Animal Science and Technology, Southwest University, Chongqing 400715, China
2.
Chongqing Key Laboratory of Forage & Herbivore, Chongqing 400715, China
3.
Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
4.
College of Animal Science, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia 010018, China
5.
School of Animal Science & Biotechnology, Mongolian University of Life Sciences, Zaisan, Khan-uul, Ulaanbaatar 17024, Mongolia
6.
Animal Science and Technology College, Jilin Agriculture University, Changchun, Jilin 130118, China
7.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
8.
Key Lab of Agricultural Animal, Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong, Agricultural University, Wuhan, Hubei 430070, China
9.
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong 266109, China
10.
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, Inner Mongolia 010070, China
11.
Department of Zoology, Pachhunga University College, Mizoram University, Aizawl 796001, India

All data in this article can be downloaded from the NCBI (PRJNA843937), GSA (PRJCA013855), and Science Data Bank databases (DOI:10.57760/sciencedb.j00139.00043).
#Authors contributed equally to this work

Funds: This work was supported by the National Natural Science Foundation of China (32272834)

More Information

Corresponding author: E-mail: eguangxin@126.com
Received Date: 2022-11-25
Accepted Date: 2023-02-06

Published Online: 2023-02-07

Publish Date: 2023-03-18

Abstract

Abstract

The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance. However, the genetic basis of muscle development in the Boer goat remains obscure. In this study, we identified specific structural variants in the Boer goat based on genome-wide selection signals and analyzed the basis of the molecular heredity of related candidate genes in muscle development. A total of 9 959 autosomal copy number variations (CNVs) were identified through selection signal analysis in 127 goat genomes. Specifically, we confirmed that the highest signal CNV (HSV) was a chromosomal arrangement containing an approximately 1.11 Mb (CHIR17: 60062304–61171840 bp) duplicated fragment inserted in reverse orientation and a 5 362 bp deleted region (CHIR17:60145940–60151302 bp) with overlapping genes (e.g., ARHGAP10, NR3C2, EDNRA, PRMT9, and TMEM184C). The homozygous duplicated HSV genotype (+/+) was found in 96% of Boer goats but was not detected in Eurasian goats and was only detected in 4% of indigenous African goats. The expression network of three candidate genes (ARHGAP10, NR3C2, and EDNRA) regulating dose transcription was constructed by RNA sequencing. Results indicated that these genes were involved in the proliferation and differentiation of skeletal muscle satellite cells (SMSCs) and their overexpression significantly increased the expression of SAA3. The HSV of the Boer goat contributed to superior skeletal muscle growth via the dose effects of overlapping genes.
- Boer goat,
- CNV,
- Muscle development,
- SMSCs

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References(90)

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