Volume 43 Issue 6
Nov.  2022
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Yan Zhou, Tian-Xiu Qiu, Yang Hu, Lei Liu, Jiong Chen. Antiviral effects of natural small molecules on aquatic rhabdovirus by interfering with early viral replication. Zoological Research, 2022, 43(6): 966-976. doi: 10.24272/j.issn.2095-8137.2022.234
Citation: Yan Zhou, Tian-Xiu Qiu, Yang Hu, Lei Liu, Jiong Chen. Antiviral effects of natural small molecules on aquatic rhabdovirus by interfering with early viral replication. Zoological Research, 2022, 43(6): 966-976. doi: 10.24272/j.issn.2095-8137.2022.234

Antiviral effects of natural small molecules on aquatic rhabdovirus by interfering with early viral replication

doi: 10.24272/j.issn.2095-8137.2022.234
Supplementary data to this article can be found online.
The authors declare that they have no competing interests.
J.C. and L.L. designed the study. Y.Z., T.X.Q., and Y.H. performed the experiments. Y.Z. drafted the manuscript with assistance from L.L. All authors read and approved the final version of the manuscript.
Funds:  This work was supported by the National Natural Science Foundation of China (31902410), Natural Science Foundation of Zhejiang Province (LQ22C190002), Natural Science Foundation of Ningbo City (2021J117), Program of Science and Technology Department of Ningbo City (2021S058), One Health Interdisciplinary Research Project of Ningbo University (HZ202201), and Zhejiang Xinmiao Talents Programs (2022R405B066)
More Information
  • Corresponding author: E-mail: liulei2@nbu.edu.cnjchen1975@163.com
  • Received Date: 2022-09-11
  • Accepted Date: 2022-10-10
  • Published Online: 2022-10-14
  • Publish Date: 2022-11-18
  • Spring viremia of carp virus (SVCV) is globally widespread and poses a serious threat to aquatic ecology and aquaculture due to its broad host range. To develop effective agents to control SVCV infection, we selected 16 naturally active small molecules to assess their anti-SVCV activity. Notably, dihydroartemisinin (DHA) (100 µmol/L) and (S, S)-(+)-tetrandrine (TET) (16 µmol/L) exhibited high antiviral effects in epithelioma papulosum cyprinid (EPC) cells, with inhibitory rates of 70.11% and 73.54%, respectively. The possible antiviral mechanisms were determined as follows: 1. Pre-incubation with DHA and TET decreased viral particle infectivity in fish cells, suggesting that horizontal transmission of SVCV in the aquatic environment was disrupted; 2. Although neither had an effect on viral adhesion, TET (but not DHA) interfered with SVCV entry into host cells (>80%), suggesting that TET may have an antiviral function in early viral replication. For in vivo study, both agents enhanced the survival rate of SVCV-infected zebrafish by 53.3%, significantly decreased viral load, and modulated the expression of antiviral-related genes, indicating that DHA and TET may stimulate the host innate immune response to prevent viral infection. Overall, our findings indicated that DHA and TET had positive effects on suppressing SVCV infection by affecting early-stage viral replication, thus holding great potential as immunostimulants to reduce the risk of aquatic rhabdovirus disease outbreaks.
  • Supplementary data to this article can be found online.
    The authors declare that they have no competing interests.
    J.C. and L.L. designed the study. Y.Z., T.X.Q., and Y.H. performed the experiments. Y.Z. drafted the manuscript with assistance from L.L. All authors read and approved the final version of the manuscript.
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