Volume 43 Issue 6
Nov.  2022
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Article Contents
Biao Yuan, Ling-Min Zhao, Zhi-Xia Zhuang, Xiao-Ru Wang, Qi Fu, Hua-Bin Huang, Li-Xing Huang, Ying-Xue Qin, Qing-Pi Yan. Transcriptomic and metabolomic insights into the role of the flgK gene in the pathogenicity of Pseudomonas plecoglossicida to orange-spotted grouper (Epinephelus coioides). Zoological Research, 2022, 43(6): 952-965. doi: 10.24272/j.issn.2095-8137.2022.216
Citation: Biao Yuan, Ling-Min Zhao, Zhi-Xia Zhuang, Xiao-Ru Wang, Qi Fu, Hua-Bin Huang, Li-Xing Huang, Ying-Xue Qin, Qing-Pi Yan. Transcriptomic and metabolomic insights into the role of the flgK gene in the pathogenicity of Pseudomonas plecoglossicida to orange-spotted grouper (Epinephelus coioides). Zoological Research, 2022, 43(6): 952-965. doi: 10.24272/j.issn.2095-8137.2022.216

Transcriptomic and metabolomic insights into the role of the flgK gene in the pathogenicity of Pseudomonas plecoglossicida to orange-spotted grouper (Epinephelus coioides)

doi: 10.24272/j.issn.2095-8137.2022.216
The RNA-seq data were submitted to the Science Data Bank database (DOI: 10.57760/sciencedb.02438), National Center for Biotechnology Information (GenBank SRA database under accession numbers SRP374205 (flgK-RNAi strain-infected group), SRP370956 (NZBD9 strain-infected group), and SRP370960 (PBS-injected group)), and National Genomics Data Center under accession number CRA007966. The metabolome data were deposited in the China National GeneBank Database under accession number CNP0002964.
Supplementary data to this article can be found online.
The authors declare that they have no competing interests.
Q.P.Y., Z.X.Z., and X.R.W. designed the study. Q.P.Y. supervised the experiments. B.Y., L.M.Z., Q.F., and H.B.H. performed the experiments. L.X.H. and Y.X.Q. performed transcriptome and metabolome data analysis. B.Y. wrote the manuscript with the other authors’ input. B.Y. submitted data to GenBank. Q.P.Y, Z.X.Z., and X.R.W revised the manuscript. All authors read and approved the final version of the manuscript.
Funds:  This work was supported by the National Natural Science Foundation of China (31972836), Natural Science Foundation of Fujian Province (2021J01828), and Open Fund of Fujian Province Key Laboratory of Special Aquatic Formula Feed (TMKJZ2101)
More Information
  • Corresponding author: E-mail: yanqp@jmu.edu.cn
  • Received Date: 2022-08-01
  • Accepted Date: 2022-09-26
  • Published Online: 2022-09-26
  • Publish Date: 2022-11-18
  • Pseudomonas plecoglossicida is the pathogen responsible for visceral white spot disease in large yellow croaker (Larimichthys crocea) and orange-spotted grouper (Epinephelus coioides). Previously, RNA sequencing showed that P. plecoglossicida flgK gene expression was significantly up-regulated in orange-spotted grouper spleens during infection. To explore the role of flgK in P. plecoglossicida pathogenicity, RNA interference (RNAi) was performed to silence the P. plecoglossicida flgK gene, and the mutant (flgK-RNAi strain) with the best silencing efficiency (89.40%) was chosen for further study. Results showed that flgK gene silencing significantly attenuated P. plecoglossicida motility, adhesion, and biofilm formation. Compared to those fish infected with the wild-type strain of P. plecoglossicida, orange-spotted grouper infected with the flgK-RNAi strain showed a 55% increase in the survival rate and a one-day delay in time of first death, with fewer pathogens in the spleen and fewer white spots on the spleen surface. RNAi of flgK significantly affected the transcriptome and metabolome of the spleen in infected orange-spotted grouper. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the C-type lectin receptor signaling pathway was the most significantly changed immune-related pathway and the mitogen-activated protein kinase (MAPK) signaling pathway was related to multiple immune-related pathways. Furthermore, arginine biosynthesis and glycerophospholipid metabolism were the most significantly changed metabolism-related pathways. These findings suggest that flgK is a virulence gene of P. plecoglossicida. Furthermore, flgK appears to be involved in the regulation of motility, adhesion, and biofilm formation in P. plecoglossicida, as well as in the regulation of inflammatory and immune responses of orange-spotted grouper to P. plecoglossicida infection.
  • The RNA-seq data were submitted to the Science Data Bank database (DOI: 10.57760/sciencedb.02438), National Center for Biotechnology Information (GenBank SRA database under accession numbers SRP374205 (flgK-RNAi strain-infected group), SRP370956 (NZBD9 strain-infected group), and SRP370960 (PBS-injected group)), and National Genomics Data Center under accession number CRA007966. The metabolome data were deposited in the China National GeneBank Database under accession number CNP0002964.
    Supplementary data to this article can be found online.
    The authors declare that they have no competing interests.
    Q.P.Y., Z.X.Z., and X.R.W. designed the study. Q.P.Y. supervised the experiments. B.Y., L.M.Z., Q.F., and H.B.H. performed the experiments. L.X.H. and Y.X.Q. performed transcriptome and metabolome data analysis. B.Y. wrote the manuscript with the other authors’ input. B.Y. submitted data to GenBank. Q.P.Y, Z.X.Z., and X.R.W revised the manuscript. All authors read and approved the final version of the manuscript.
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