Maternal sleep deprivation induces gut microbial dysbiosis and neuroinflammation in offspring rats

Zheng-Yu Yao; Xiao-Huan Li; Li Zuo; Qian Xiong; Wen-Ting He; Dong-Xu Li; Zhi-Fang Dong

doi:10.24272/j.issn.2095-8137.2022.023

Volume 43 Issue 3

May 2022

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Article Navigation > Zoological Research > 2022 > 43(3): 380-390

Zheng-Yu Yao, Xiao-Huan Li, Li Zuo, Qian Xiong, Wen-Ting He, Dong-Xu Li, Zhi-Fang Dong. Maternal sleep deprivation induces gut microbial dysbiosis and neuroinflammation in offspring rats. Zoological Research, 2022, 43(3): 380-390. doi: 10.24272/j.issn.2095-8137.2022.023

Citation:

Zheng-Yu Yao, Xiao-Huan Li, Li Zuo, Qian Xiong, Wen-Ting He, Dong-Xu Li, Zhi-Fang Dong. Maternal sleep deprivation induces gut microbial dysbiosis and neuroinflammation in offspring rats. Zoological Research, 2022, 43(3): 380-390. doi: 10.24272/j.issn.2095-8137.2022.023

Citation:

Zheng-Yu Yao, Xiao-Huan Li, Li Zuo, Qian Xiong, Wen-Ting He, Dong-Xu Li, Zhi-Fang Dong. Maternal sleep deprivation induces gut microbial dysbiosis and neuroinflammation in offspring rats. Zoological Research, 2022, 43(3): 380-390. doi: 10.24272/j.issn.2095-8137.2022.023

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Maternal sleep deprivation induces gut microbial dysbiosis and neuroinflammation in offspring rats

doi: 10.24272/j.issn.2095-8137.2022.023

1.
Pediatric Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Children’s Hospital of Chongqing Medical University, Chongqing 400014, China

Funds: This work was supported by the National Natural Science Foundation of China (82071395, 91749116), Natural Science Foundation of Chongqing (cstc2021ycjh-bgzxm0186, cstc2020jcyj-zdxmX0004), Science and Technology Research Program of Chongqing Municipal Education Commission (KJZD-K201900403), and Innovation Research Group at Institutions of Higher Education in Chongqing (CXQTP19034)

More Information

Corresponding author: E-mail: zfdong@cqmu.edu.cn
Received Date: 2022-02-24
Accepted Date: 2022-03-31

Published Online: 2022-03-31

Publish Date: 2022-05-18

Abstract

Abstract

Maternal sleep deprivation (MSD) is a global public health problem that affects the physical and mental development of pregnant women and their newborns. The latest research suggests that sleep deprivation (SD) disrupts the gut microbiota, leading to neuroinflammation and psychological disturbances. However, it is unclear whether MSD affects the establishment of gut microbiota and neuroinflammation in the newborns. In the present study, MSD was performed on pregnant Sprague-Dawley rats in the third trimester of pregnancy (gestational days 15–21), after which intestinal contents and brain tissues were collected from offspring at different postnatal days (P1, P7, P14, and P56). Based on microbial profiling, microbial diversity and richness increased in pregnant rats subjected to MSD, as reflected by the significant increase in the phylum Firmicutes. In addition, microbial dysbiosis marked by abundant Firmicutes bacteria was observed in the MSD offspring. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) showed that the expression levels of proinflammatory cytokines interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) were significantly higher in the MSD offspring at adulthood (P56) than in the control group. Through Spearman correlation analysis, IL-1β and TNF-α were also shown to be positively correlated with Ruminococcus_1 and Ruminococcaceae_UCG-005 at P56, which may determine the microbiota-host interactions in MSD-related neuroinflammation. Collectively, these results indicate that MSD changes maternal gut microbiota and affects the establishment of neonatal gut microbiota, leading to neuroinflammation in MSD offspring. Therefore, understanding the role of gut microbiota during physiological development may provide potential interventions for cognitive dysfunction in MSD-impacted offspring.
- Maternal sleep deprivation,
- Gut microbiota,
- Neuroinflammation,
- Gut-brain axis,
- Cognitive function,
- Firmicutes

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References(54)

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