Volume 43 Issue 3
May  2022
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Yi-Fang Lu, Qian Liu, Kai-Qiang Liu, Hong-Yan Wang, Cheng-Hua Li, Qian Wang, Chang-Wei Shao. Identification of global alternative splicing and sex-specific splicing via comparative transcriptome analysis of gonads of Chinese tongue sole (Cynoglossus semilaevis). Zoological Research, 2022, 43(3): 319-330. doi: 10.24272/j.issn.2095-8137.2021.406
Citation: Yi-Fang Lu, Qian Liu, Kai-Qiang Liu, Hong-Yan Wang, Cheng-Hua Li, Qian Wang, Chang-Wei Shao. Identification of global alternative splicing and sex-specific splicing via comparative transcriptome analysis of gonads of Chinese tongue sole (Cynoglossus semilaevis). Zoological Research, 2022, 43(3): 319-330. doi: 10.24272/j.issn.2095-8137.2021.406

Identification of global alternative splicing and sex-specific splicing via comparative transcriptome analysis of gonads of Chinese tongue sole (Cynoglossus semilaevis)

doi: 10.24272/j.issn.2095-8137.2021.406
Funds:  This work was supported by the National Natural Science Foundation of China (31722058, 31802275, and 31472269); National Key R&D Program of China (2018YFD0900301); AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology (2017ASTCP-ES06); Taishan Scholar Project Fund of Shandong of China to C.W.S.; National Ten-Thousands Talents Special Support Program to C.W.S.; Central Public-Interest Scientific Institution Basal Research Fund, CAFS (2020TD19); Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS (20603022021018); China Agriculture Research System (CARS-47-G03); and Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals, Guangdong Ocean University (KFKT2019ZD03).
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  • Chinese tongue sole (Cynoglossus semilaevis) is an economically important marine fish species with a ZZ/ZW sex determination mechanism, which can be influenced by temperature. Alternative splicing (AS) is an important mechanism regulating the expression of genes related to sex determination and gonadal differentiation, but has rarely been reported in fish. In this study, to explore the molecular regulatory mechanisms of sex determination and gonadal differentiation, we combined isoform and RNA sequencing (Iso-Seq and RNA-Seq) to perform transcriptome profiling of male and female gonads in C. semilaevis. In total, 81 883 and 32 341 full-length transcripts were obtained in males and females, respectively. A total of 8 279 AS genes were identified, including 2 639 genes showing differential AS (DAS) between males and females. Many intersecting DAS genes and differentially expressed genes (DEGs) were enriched in the meiotic cell cycle pathway, and genes related to gonadal differentiation, such as esrrb and wt1a, were found to have sex-specific isoforms. Thus, this study revealed AS events in the gonadal transcriptomes of male and female C. semilaevis, described the characteristics of active transcription in the testes, and identified candidate genes for studying the regulatory mechanisms of AS during gonadal differentiation.
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