Chromosome-level genome assembly of the dotted gizzard shad (Konosirus punctatus) provides insights into its adaptive evolution

Konosirus punctatus is an economically important marine fishery resource and is widely distributed from the Indian to Pacific oceans. It is a good non-model species for genetic studies on salinity and temperature adaptation. However, a high-quality reference genome has not yet been reported. Here, an 800.00 Mb high-quality chromosome-level genome with a contig N50 length of 2.14 Mb was assembled using Illumina, Pacific Biosciences, and Hi-C sequencing technology. The assembled sequences were anchored to 24 pseudochromosomes by the Hi-C data. In total, 24 298 protein-coding genes were predicted, 91.08% of which were successfully annotated with putative functions. Furthermore, 587 putative genes were identified as being under positive selection. This new high-quality K. punctatus reference genome provides a fundamental resource for a deeper understanding of temperature and salinity adaptation and species conservation.