Volume 42 Issue 5
Sep.  2021
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Kuan-Xiang Sun, Xiao-Yan Jiang, Xiao Li, Yu-Jing Su, Ju-Lin Wang, Lin Zhang, Ye-Ming Yang, Xian-Jun Zhu. Deletion of phosphatidylserine flippase β-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration. Zoological Research, 2021, 42(5): 650-659. doi: 10.24272/j.issn.2095-8137.2021.195
Citation: Kuan-Xiang Sun, Xiao-Yan Jiang, Xiao Li, Yu-Jing Su, Ju-Lin Wang, Lin Zhang, Ye-Ming Yang, Xian-Jun Zhu. Deletion of phosphatidylserine flippase β-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration. Zoological Research, 2021, 42(5): 650-659. doi: 10.24272/j.issn.2095-8137.2021.195

Deletion of phosphatidylserine flippase β-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration

doi: 10.24272/j.issn.2095-8137.2021.195
#Authors contributed equally to this work
Funds:  This study was supported by the National Natural Science Foundation of China (81770950, 81970841), Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciences (2019-12M-5-032), and Department of Science and Technology of Sichuan Province (21ZDYF4279, 2020JDZH0026, 2021JDZH0022)
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  • Corresponding author: E-mail: xjzhu@uestc.edu.cn
  • Received Date: 2021-08-03
  • Accepted Date: 2021-08-30
  • Published Online: 2021-08-31
  • Publish Date: 2021-09-18
  • Phosphatidylserine (PS) is distributed asymmetrically in the plasma membrane of eukaryotic cells. Phosphatidylserine flippase (P4-ATPase) transports PS from the outer leaflet of the lipid bilayer to the inner leaflet of the membrane to maintain PS asymmetry. The β subunit TMEM30A is indispensable for transport and proper function of P4-ATPase. Previous studies have shown that the ATP11A and TMEM30A complex is the molecular switch for myotube formation. However, the role of Tmem30a in skeletal muscle regeneration remains elusive. In the current study, Tmem30a was highly expressed in the tibialis anterior (TA) muscles of dystrophin-null (mdx) mice and BaCl2-induced muscle injury model mice. We generated a satellite cell (SC)-specific Tmem30a conditional knockout (cKO) mouse model to investigate the role of Tmem30a in skeletal muscle regeneration. The regenerative ability of cKO mice was evaluated by analyzing the number and diameter of regenerated SCs after the TA muscles were injured by BaCl2-injection. Compared to the control mice, the cKO mice showed decreased Pax7+ and MYH3+ SCs, indicating diminished SC proliferation, and decreased expression of muscular regulatory factors (MYOD and MYOG), suggesting impaired myoblast proliferation in skeletal muscle regeneration. Taken together, these results demonstrate the essential role of Tmem30a in skeletal muscle regeneration.
  • #Authors contributed equally to this work
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