Potential aquatic environmental risks of trifloxystrobin: Enhancement of virus susceptibility in zebrafish through initiation of autophagy

Huan Wang; Tian-Xiu Qiu; Jian-Fei Lu; Han-Wei Liu; Ling Hu; Lei Liu; Jiong Chen

doi:10.24272/j.issn.2095-8137.2021.056

Volume 42 Issue 3

May 2021

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Article Navigation > Zoological Research > 2021 > 42(3): 339-349

Huan Wang, Tian-Xiu Qiu, Jian-Fei Lu, Han-Wei Liu, Ling Hu, Lei Liu, Jiong Chen. Potential aquatic environmental risks of trifloxystrobin: Enhancement of virus susceptibility in zebrafish through initiation of autophagy. Zoological Research, 2021, 42(3): 339-349. doi: 10.24272/j.issn.2095-8137.2021.056

Citation:

Huan Wang, Tian-Xiu Qiu, Jian-Fei Lu, Han-Wei Liu, Ling Hu, Lei Liu, Jiong Chen. Potential aquatic environmental risks of trifloxystrobin: Enhancement of virus susceptibility in zebrafish through initiation of autophagy. Zoological Research, 2021, 42(3): 339-349. doi: 10.24272/j.issn.2095-8137.2021.056

Citation:

Huan Wang, Tian-Xiu Qiu, Jian-Fei Lu, Han-Wei Liu, Ling Hu, Lei Liu, Jiong Chen. Potential aquatic environmental risks of trifloxystrobin: Enhancement of virus susceptibility in zebrafish through initiation of autophagy. Zoological Research, 2021, 42(3): 339-349. doi: 10.24272/j.issn.2095-8137.2021.056

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Potential aquatic environmental risks of trifloxystrobin: Enhancement of virus susceptibility in zebrafish through initiation of autophagy

doi: 10.24272/j.issn.2095-8137.2021.056

Huan Wang^{1, 2, 3},
Tian-Xiu Qiu^{1, 2, 3},
Jian-Fei Lu^{1, 2, 3},
Han-Wei Liu⁴,
Ling Hu⁴,
Lei Liu^{1, 2, 3
,
,},
Jiong Chen^{1, 2, 3
,
,}

1.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang 315211, China
2.
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Meishan Campus, Ningbo University, Ningbo, Zhejiang 315832, China
3.
Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Meishan Campus, Ningbo University, Ningbo, Zhejiang 315832, China
4.
Ningbo Customs District Technology Center, Ningbo, Zhejiang 315832, China

Funds: This work was supported by the National Natural Science Foundation of China (31902410), Program of State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products (KF20200106), Natural Science Foundation of Zhejiang Province (LY21C190002), and Foundation of Ningbo City of China (202003N4120)

More Information

Corresponding author: E-mail: liulei2@nbu.edu.cn; jchen1975@163.com
Received Date: 2021-02-20
Accepted Date: 2021-04-06

Published Online: 2021-05-08

Publish Date: 2021-05-18

Abstract

Abstract

Chronic pollution in aquatic ecosystems can lead to many adverse effects, including a greater susceptibility to pathogens among resident biota. Trifloxystrobin (TFS) is a strobilurin fungicide widely used in Asia to control soybean rust. However, it has the potential to enter aquatic ecosystems, where it may impair fish resistance to viral infections. To explore the potential environmental risks of TFS, we characterized the antiviral capacities of fish chronically exposed to TFS and subsequently infected with spring viraemia of carp virus (SVCV). Although TFS exhibited no significant cytotoxicity at the tested environmental concentrations during viral challenge, SVCV replication increased significantly in a time-dependent manner within epithelioma papulosum cyprini (EPC) cells and zebrafish exposed to 25 μg/L TFS. Results showed that the highest viral load was more than 100-fold that of the controls. Intracellular biochemical assays indicated that autophagy was induced by TFS, and associated changes included an increase in autophagosomes, conversion of LC3-II, accumulation of Beclin-1, and degradation of P62 in EPC cells and zebrafish. In addition, TFS markedly decreased the expression and phosphorylation of mTOR, indicating that activation of TFS may be associated with the mTOR-mediated autophagy pathway. This study provides new insights into the mechanism of the immunosuppressive effects of TFS on non-target aquatic hosts and suggests that the existence of TFS in aquatic environments may contribute to outbreaks of viral diseases.
- Trifloxystrobin,
- Autophagy,
- SVCV,
- Chronic toxicity,
- Susceptibility

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References(56)

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