Lower respiratory tract samples are reliable for severe acute respiratory syndrome coronavirus 2 nucleic acid diagnosis and animal model study

Ren-Rong Tian; Cui-Xian Yang; Mi Zhang; Xiao-Li Feng; Rong-Hua Luo; Zi-Lei Duan; Jian-Jian Li; Jia-Fa Liu; Dan-Dan Yu; Ling Xu; Hong-Yi Zheng; Ming-Hua Li; Hong-Li Fan; Jia-Li Wang; Xing-Qi Dong; Yong-Tang Zheng

doi:10.24272/j.issn.2095-8137.2020.329

Volume 42 Issue 2

Mar. 2021

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Article Navigation > Zoological Research > 2021 > 42(2): 161-169

Ren-Rong Tian, Cui-Xian Yang, Mi Zhang, Xiao-Li Feng, Rong-Hua Luo, Zi-Lei Duan, Jian-Jian Li, Jia-Fa Liu, Dan-Dan Yu, Ling Xu, Hong-Yi Zheng, Ming-Hua Li, Hong-Li Fan, Jia-Li Wang, Xing-Qi Dong, Yong-Tang Zheng. Lower respiratory tract samples are reliable for severe acute respiratory syndrome coronavirus 2 nucleic acid diagnosis and animal model study. Zoological Research, 2021, 42(2): 161-169. doi: 10.24272/j.issn.2095-8137.2020.329

Citation:

Ren-Rong Tian, Cui-Xian Yang, Mi Zhang, Xiao-Li Feng, Rong-Hua Luo, Zi-Lei Duan, Jian-Jian Li, Jia-Fa Liu, Dan-Dan Yu, Ling Xu, Hong-Yi Zheng, Ming-Hua Li, Hong-Li Fan, Jia-Li Wang, Xing-Qi Dong, Yong-Tang Zheng. Lower respiratory tract samples are reliable for severe acute respiratory syndrome coronavirus 2 nucleic acid diagnosis and animal model study. Zoological Research, 2021, 42(2): 161-169. doi: 10.24272/j.issn.2095-8137.2020.329

Citation:

Ren-Rong Tian, Cui-Xian Yang, Mi Zhang, Xiao-Li Feng, Rong-Hua Luo, Zi-Lei Duan, Jian-Jian Li, Jia-Fa Liu, Dan-Dan Yu, Ling Xu, Hong-Yi Zheng, Ming-Hua Li, Hong-Li Fan, Jia-Li Wang, Xing-Qi Dong, Yong-Tang Zheng. Lower respiratory tract samples are reliable for severe acute respiratory syndrome coronavirus 2 nucleic acid diagnosis and animal model study. Zoological Research, 2021, 42(2): 161-169. doi: 10.24272/j.issn.2095-8137.2020.329

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Lower respiratory tract samples are reliable for severe acute respiratory syndrome coronavirus 2 nucleic acid diagnosis and animal model study

doi: 10.24272/j.issn.2095-8137.2020.329

Ren-Rong Tian^{1, 2, #},
Cui-Xian Yang^{3, #},
Mi Zhang³,
Xiao-Li Feng^{2, #},
Rong-Hua Luo^{1, 2},
Zi-Lei Duan^{1, 2},
Jian-Jian Li³,
Jia-Fa Liu³,
Dan-Dan Yu^{1, 2},
Ling Xu^{1, 2},
Hong-Yi Zheng^{1, 2},
Ming-Hua Li²,
Hong-Li Fan³,
Jia-Li Wang³,
Xing-Qi Dong^{3
,
,},
Yong-Tang Zheng^{1, 2
,
,}

1.
Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
2.
Center for Biosafety Mega-Science, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
3.
Yunnan Infectious Diseases Hospital, Kunming, Yunnan 650301, China

#Authors contributed equally to this work

Funds: This work was supported in part by the National Key R&D Program of China (2020YFC0842000 and 2020YFC0847000 to Y.T.Z.), Yunnan Provincial Major Science Technology Project (202003AC100008 to X.Q.D.), and Key Research Program of the Chinese Academy of Sciences (KJZD-SW-L11 to Y.T.Z.)

More Information

Corresponding author: E-mail: dongxq8001@126.com; zhengyt@mail.kiz.ac.cn
Received Date: 2020-11-10
Accepted Date: 2021-02-07

Published Online: 2021-02-07

Publish Date: 2021-03-18

Abstract

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease 2019 (COVID-19) continue to impact countries worldwide. At present, inadequate diagnosis and unreliable evaluation systems hinder the implementation and development of effective prevention and treatment strategies. Here, we conducted a horizontal and longitudinal study comparing the detection rates of SARS-CoV-2 nucleic acid in different types of samples collected from COVID-19 patients and SARS-CoV-2-infected monkeys. We also detected anti-SARS-CoV-2 antibodies in the above clinical and animal model samples to identify a reliable approach for the accurate diagnosis of SARS-CoV-2 infection. Results showed that, regardless of clinical symptoms, the highest detection levels of viral nucleic acid were found in sputum and tracheal brush samples, resulting in a high and stable diagnosis rate. Anti-SARS-CoV-2 immunoglobulin M (IgM) and G (IgG) antibodies were not detected in 6.90% of COVID-19 patients. Furthermore, integration of nucleic acid detection results from the various sample types did not improve the diagnosis rate. Moreover, dynamic changes in SARS-CoV-2 viral load were more obvious in sputum and tracheal brushes than in nasal and throat swabs. Thus, SARS-CoV-2 nucleic acid detection in sputum and tracheal brushes was the least affected by infection route, disease progression, and individual differences. Therefore, SARS-CoV-2 nucleic acid detection using lower respiratory tract samples alone is reliable for COVID-19 diagnosis and study.
- COVID-19,
- SARS-CoV-2,
- Diagnosis,
- Animal model

#Authors contributed equally to this work

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References(30)

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