Fluoxetine ameliorates depressive symptoms by regulating lncRNA expression in the mouse hippocampus

Chuan-Ling Zhang; Yi-Jia Li; Shuang Lu; Ting Zhang; Rui Xiao; Huai-Rong Luo

doi:10.24272/j.issn.2095-8137.2020.294

Volume 42 Issue 1

Jan. 2021

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Chuan-Ling Zhang, Yi-Jia Li, Shuang Lu, Ting Zhang, Rui Xiao, Huai-Rong Luo. Fluoxetine ameliorates depressive symptoms by regulating lncRNA expression in the mouse hippocampus. Zoological Research, 2021, 42(1): 28-42. doi: 10.24272/j.issn.2095-8137.2020.294

Citation:

Chuan-Ling Zhang, Yi-Jia Li, Shuang Lu, Ting Zhang, Rui Xiao, Huai-Rong Luo. Fluoxetine ameliorates depressive symptoms by regulating lncRNA expression in the mouse hippocampus. Zoological Research, 2021, 42(1): 28-42. doi: 10.24272/j.issn.2095-8137.2020.294

Citation:

Chuan-Ling Zhang, Yi-Jia Li, Shuang Lu, Ting Zhang, Rui Xiao, Huai-Rong Luo. Fluoxetine ameliorates depressive symptoms by regulating lncRNA expression in the mouse hippocampus. Zoological Research, 2021, 42(1): 28-42. doi: 10.24272/j.issn.2095-8137.2020.294

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Fluoxetine ameliorates depressive symptoms by regulating lncRNA expression in the mouse hippocampus

doi: 10.24272/j.issn.2095-8137.2020.294

Chuan-Ling Zhang^{1, 2, 3},
Yi-Jia Li⁴,
Shuang Lu⁴,
Ting Zhang⁴,
Rui Xiao^{4
,
,},
Huai-Rong Luo^{1, 5
,
,}

1.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medical Chemistry, Kunming Institute of Botany, the Chinese Academy of Sciences, Kunming, Yunnan 650201, China
2.
School of Pharmacy, Inner Mongolia Medical University, Huhhot, Inner Mongolia 010110, China
3.
University of the Chinese Academy of Sciences, Beijing 100049, China
4.
Key Laboratory of Molecular Pathology, Inner Mongolia Medical University, Huhhot, Inner Mongolia 010059, China
5.
Key Laboratory for Aging and Regenerative Medicine, Department of Pharmacology, School of Pharmacy, Southwestern Medical University, Luzhou, Sichuan 646000, China

Funds: This work was supported by the Scientific Research Projects of Universities in Inner Mongolia Autonomous Region (NJZY111) and Natural Scientific Research Projects of Inner Mongolia Autonomous Region (2020MS03060)

More Information

Corresponding author: E-mail: xiaorui79@hotmail.com; lhr@swmu.edu.cn
Received Date: 2020-11-25
Accepted Date: 2021-01-06

Published Online: 2021-01-08

Publish Date: 2021-01-18

Abstract

Abstract

Depression is a prevalent mental disorder that is associated with aging and contributes to increased mortality and morbidity. The overall prevalence of geriatric depression with clinically significant symptoms is currently on the rise. Recent studies have demonstrated that altered expressions of long non-coding RNAs (lncRNAs) in the brain affect neurodevelopment and manifest modulating functions during the depression. However, most lncRNAs have not yet been studied. Herein, we analyzed the transcriptome of dysregulated lncRNAs to reveal their expressions in a mouse model exhibiting depressive-like behaviors, as well as their corresponding response following antidepressant fluoxetine treatment. A chronic unpredictable mild stress (CUMS) mouse model was applied. A six-week fluoxetine intervention in CUMS-induced mice attenuated depressive-like behaviors. In addition, differential expression analysis of lncRNAs was performed following RNA-sequencing. A total of 282 lncRNAs (134 up-regulated and 148 down-regulated) were differentially expressed in CUMS-induced mice relative to non-stressed counterparts (P<0.05). Moreover, 370 differentially expressed lncRNAs were identified in CUMS-induced mice after fluoxetine intervention. Gene Ontology (GO) analyses showed an association between significantly dysregulated lncRNAs and protein binding, oxygen binding, and transport activity, while the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that these dysregulated lncRNAs might be involved in inflammatory response pathways. Fluoxetine effectively ameliorated the symptoms of depression in CUMS-induced mice by regulating the expression of lncRNAs in the hippocampus. The findings herein provide valuable insights into the potential mechanism underlying depression in elderly people.
- Depression,
- Elderly,
- Fluoxetine,
- LncRNA,
- Chronic unpredictable mild stress

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References(57)

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