A tree shrew model for steroid-associated osteonecrosis

Qi Chen; Zhao-Xia Ma; Li-Bin Xia; Zhen-Ni Ye; Bao-Ling Liu; Tie-Kun Ma; Peng-Fei Bao; Xing-Fei Wu; Cong-Tao Yu; Dai-Ping Ma; Yuan-Yuan Han; Wen-Guang Wang; De-Xuan Kuang; Jie-Jie Dai; Rong-Ping Zhang; Min Hu; Hong Shi; Wen-Lin Wang; Yan-Jiao Li

doi:10.24272/j.issn.2095-8137.2020.061

Volume 41 Issue 5

Sep. 2020

Turn off MathJax

Article Contents

Article Navigation > Zoological Research > 2020 > 41(5): 564-568

Qi Chen, Zhao-Xia Ma, Li-Bin Xia, Zhen-Ni Ye, Bao-Ling Liu, Tie-Kun Ma, Peng-Fei Bao, Xing-Fei Wu, Cong-Tao Yu, Dai-Ping Ma, Yuan-Yuan Han, Wen-Guang Wang, De-Xuan Kuang, Jie-Jie Dai, Rong-Ping Zhang, Min Hu, Hong Shi, Wen-Lin Wang, Yan-Jiao Li. A tree shrew model for steroid-associated osteonecrosis. Zoological Research, 2020, 41(5): 564-568. doi: 10.24272/j.issn.2095-8137.2020.061

Citation:

Qi Chen, Zhao-Xia Ma, Li-Bin Xia, Zhen-Ni Ye, Bao-Ling Liu, Tie-Kun Ma, Peng-Fei Bao, Xing-Fei Wu, Cong-Tao Yu, Dai-Ping Ma, Yuan-Yuan Han, Wen-Guang Wang, De-Xuan Kuang, Jie-Jie Dai, Rong-Ping Zhang, Min Hu, Hong Shi, Wen-Lin Wang, Yan-Jiao Li. A tree shrew model for steroid-associated osteonecrosis. Zoological Research, 2020, 41(5): 564-568. doi: 10.24272/j.issn.2095-8137.2020.061

Citation:

Qi Chen, Zhao-Xia Ma, Li-Bin Xia, Zhen-Ni Ye, Bao-Ling Liu, Tie-Kun Ma, Peng-Fei Bao, Xing-Fei Wu, Cong-Tao Yu, Dai-Ping Ma, Yuan-Yuan Han, Wen-Guang Wang, De-Xuan Kuang, Jie-Jie Dai, Rong-Ping Zhang, Min Hu, Hong Shi, Wen-Lin Wang, Yan-Jiao Li. A tree shrew model for steroid-associated osteonecrosis. Zoological Research, 2020, 41(5): 564-568. doi: 10.24272/j.issn.2095-8137.2020.061

PDF( 2670 KB)

A tree shrew model for steroid-associated osteonecrosis

doi: 10.24272/j.issn.2095-8137.2020.061

1.
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China
2.
Yunnan Key Laboratory for Basic Research on Bone and Joint Diseases & Yunnan Stem Cell Translational Research Center, Kunming University, Kunming, Yunnan 650214, China
3.
Department Obstetrics, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, China
4.
Department of Nuclear Medicine, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, China
5.
Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan 650118, China
6.
School of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, Yunnan 650500, China
7.
Kunming Medical University, Kunming, Yunnan 650500, China

#Authors contributed equally to this work

Funds: This study was supported by the National Science and Key Technology Support Program (2014BAI01B01-07) and Science and Technology Key Projects of Kunming (2014-04-A-S-01-3072)

More Information

Corresponding author: E-mail: shih@kust.edu.cn; wenlinwang331@163.com; 391910123@qq.com
Received Date: 2020-04-24
Accepted Date: 2020-07-01

Published Online: 2020-07-29

Publish Date: 2020-09-18

Abstract

Abstract

Osteonecrosis is a common human disease in orthopedics. It is difficult to treat, and half of patients may need artificial joint replacement, resulting in a considerable economic burden and a reduction in quality of life. Hormones are one of the major causes of osteonecrosis and high doses of corticosteroids are considered the most dangerous factor. Because of the complexity of treatment, we still need a better animal model that can be widely used in drug development and testing. Tree shrews are more closely related to primates than rodents. As such, we constructed a successful tree shrew model to establish and evaluate steroid-associated osteonecrosis (SAON). We found that low-dose lipopolysaccharide (LPS) combined with high-dose methylprednisolone (MPS) over 12 weeks could be used to establish a tree shrew model with femoral head necrosis. Serum biochemical and histological analyses showed that an ideal model was obtained. Thus, this work provides a useful animal model for the study of SAON and for the optimization of treatment methods.
- Steroid-associated osteonecrosis,
- Tree shrews,
- Biochemical parameter analysis,
- Micro-CT examination,
- Histological analysis,
- Scanning electronmicroscope observation

#Authors contributed equally to this work

FullText(HTML)

References(37)

References

[1]	Amanatullah DF, Strauss EJ, Di Cesare PE. 2011. Current management options for osteonecrosis of the femoral head: part II, operative management. American Journal of Orthopedics, 40(10): E216−E225.
[2]	Beckmann R, Shaheen H, Kweider N, Ghassemi A, Fragoulis A, Hermanns-Sachweh B, et al. 2014. Enoxaparin prevents steroid-related avascular necrosis of the femoral head. The Scientific World Journal, 2014: 347813.
[3]	Bekler H, Uygur AM, Gökçe A, Beyzadeoğlu T. 2007. The effect of steroid use on the pathogenesis of avascular necrosis of the femoral head: an animal model. Acta Orthopaedica et Traumatologica Turcica, 41(1): 58−63.
[4]	Calder JDF, Pearse MF, Revell PA. 2001. The extent of osteocyte death in the proximal femur of patients with osteonecrosis of the femoral head. The Journal of Bone and Joint Surgery, 83–B(3): 419−422.
[5]	Cantatore FP, Carrozzo M, Magli DM, Pipitone V. 1991. Mononuclear cells are not involved in BGP synthesis and secretion. Clinical Rheumatology, 10(1): 28−30. doi: 10.1007/BF02208029
[6]	Chan MHM, Chan PKS, Griffith JF, Chan IHS, Lit LCW, Wong CK, et al. 2006. Steroid-induced osteonecrosis in severe acute respiratory syndrome: a retrospective analysis of biochemical markers of bone metabolism and corticosteroid therapy. Pathology, 38(3): 229−235. doi: 10.1080/00313020600696231
[7]	Cui QJ, Wang GJ, Su CC, Balian G. 1997. The Otto Aufranc Award. Lovastatin prevents steroid induced adipogenesis and osteonecrosis. Clinical Orthopaedics and Related Research, (344): 8−19.
[8]	De Ruijter J, Maas M, Janssen A, Wijburg FA. 2013. High prevalence of femoral head necrosis in Mucopolysaccharidosis type III (Sanfilippo disease): a national, observational, cross-sectional study. Molecular Genetics and Metabolism, 109(1): 49−53. doi: 10.1016/j.ymgme.2013.03.004
[9]	Fan Y, Huang ZY, Cao CC, Chen CS, Chen YX, Fan DD, et al. 2013. Genome of the Chinese tree shrew. Nature Communications, 4: 1426−1426. doi: 10.1038/ncomms2416
[10]	Fan Y, Ye MS, Zhang JY, Xu L, Yu DD, Gu TL, et al. 2019. Chromosomal level assembly and population sequencing of the Chinese tree shrew genome. Zoological Research, 40(6): 506−521. doi: 10.24272/j.issn.2095-8137.2019.063
[11]	Fazzalari NL, Kuliwaba JS, Forwood MR. 2002. Cancellous bone microdamage in the proximal femur: influence of age and osteoarthritis on damage morphology and regional distribution. Bone, 31(6): 697−702. doi: 10.1016/S8756-3282(02)00906-7
[12]	Garnero P, Hausherr E, Chapuy MC, Marcelli C, Grandjean H, Muller C, et al. 1996. Markers of bone resorption predict hip fracture in elderly women: the EPIDOS prospective study. Journal of Bone and Mineral Research, 11(10): 1531−1538.
[13]	Janke LJ, Liu CC, Vogel P, Kawedia J, Boyd KL, Funk AJ, et al. 2013. Primary epiphyseal arteriopathy in a mouse model of steroid-induced osteonecrosis. The American Journal of Pathology, 183(1): 19−25. doi: 10.1016/j.ajpath.2013.03.004
[14]	Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG. 2012. Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. Osteoarthritis and Cartilage, 20(4): 256−260. doi: 10.1016/j.joca.2012.02.010
[15]	Kim YH, Kim JS. 2004. Histologic analysis of acetabular and proximal femoral bone in patients with osteonecrosis of the femoral head. The Journal of Bone and Joint Surgery, 86(11): 2471−2474. doi: 10.2106/00004623-200411000-00017
[16]	Li CH, Yan LZ, Ban WZ, Tu Q, Wu T, Wang L, et al. 2017. Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring. Cell Research, 27(2): 241−252. doi: 10.1038/cr.2016.156
[17]	Mohamed Y, Haifa H, Datel O, Fadoua HN, Smeh BH, Mahbouba J, et al. 2014. The role of biochemical markers of bone turnover in the diagnosis of osteoporosis and predicting fracture risk. La Tunisie Medicale, 92(5): 304−310.
[18]	Mont MA, Jones LC, Hungerford DS. 2006. Nontraumatic osteonecrosis of the femoral head: ten years later. The Journal of Bone & Joint Surgery, 88(5): 1117−1132.
[19]	National Research Council Committee for the Update of the Guide for The C & Use of Laboratory A. 2011. Guide for the Care and Use of Laboratory Animals. 8th ed. Washington, DC: National Academies Press.
[20]	Petruzziello F, Fouillen L, Wadensten H, Kretz R, Andren PE, Rainer G, et al. 2012. Extensive characterization of Tupaia belangeri neuropeptidome using an integrated mass spectrometric approach. Journal of Proteome Research, 11(2): 886−896. doi: 10.1021/pr200709j
[21]	Qin L, Zhang G, Sheng H, Yeung KW, Yeung HY, Chan CW, et al. 2006. Multiple bioimaging modalities in evaluation of an experimental osteonecrosis induced by a combination of lipopolysaccharide and methylprednisolone. Bone, 39(4): 863−871. doi: 10.1016/j.bone.2006.04.018
[22]	Quan JJ, Zhou CX, Johnson NW, Francis G, Dahlstrom JE, Gao J. 2012. Molecular pathways involved in crosstalk between cancer cells, osteoblasts and osteoclasts in the invasion of bone by oral squamous cell carcinoma. Pathology, 44(3): 221−227. doi: 10.1097/PAT.0b013e3283513f3b
[23]	Ryoo S, Lee S, Jo S, Lee S, Kwak A, Kim E, et al. 2014. Effect of lipopolysaccharide (LPS) on mouse model of steroid-induced avascular necrosis in the femoral head (ANFH). Journal of Microbiology and Biotechnology, 24(3): 394−400. doi: 10.4014/jmb.1311.11057
[24]	Sun Y, Feng Y, Zhang CQ, Cheng XG, Chen SB, Ai ZS, et al. 2011. Beneficial effect of autologous transplantation of endothelial progenitor cells on steroid-induced femoral head osteonecrosis in rabbits. Cell Transplantation, 20(2): 233−243. doi: 10.3727/096368910X522234
[25]	Szulc P, Delmas PD. 2008. Biochemical markers of bone turnover: potential use in the investigation and management of postmenopausal osteoporosis. Osteoporosis International, 19(12): 1683−1704. doi: 10.1007/s00198-008-0660-9
[26]	Wang A, Ren M, Wang JC. 2018. The pathogenesis of steroid-induced osteonecrosis of the femoral head: a systematic review of the literature. Gene, 671: 103−109. doi: 10.1016/j.gene.2018.05.091
[27]	Wang YL, Ma ZX, Zheng YY, Liu BL, Bao PF, Wu XF, et al. 2019. Establishment of an osteoporosis model in tree shrews by bilateral ovariectomy and comprehensive evaluation. Experimental and Therapeutic Medicine, 17(5): 3644−3654. doi: 10.3892/etm.2019.7339
[28]	Wang YX. 1987. Taxonomic research on Burma-Chinese tree Shrew, Tupaia belangeri (Wagner), from Southern China. Zoological Research, 8(3): 213−230. (in Chinese)
[29]	Xi HB, Tao WJ, Jian ZG, Sun XF, Gong XH, Huang LX, et al. 2017. Levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head. Experimental and Therapeutic Medicine, 13(1): 69−74. doi: 10.3892/etm.2016.3964
[30]	Xiao J, Liu R, Chen CS. 2017. Tree shrew (Tupaia belangeri) as a novel laboratory disease animal model. Zoological Research, 38(3): 127−137. doi: 10.24272/j.issn.2095-8137.2017.033
[31]	Xing HJ, Jia K, He J, Shi CZ, Fang MX, Song LL, et al. 2015. Establishment of the tree shrew as an alcohol-induced Fatty liver model for the study of alcoholic liver diseases. PLoS One, 10(6): e0128253. doi: 10.1371/journal.pone.0128253
[32]	Xu JZ, Gong HP, Lu ST, Deasey MJ, Cui QJ. 2018. Animal models of steroid-induced osteonecrosis of the femoral head—a comprehensive research review up to 2018. International Orthopaedics, 42(7): 1729−1737. doi: 10.1007/s00264-018-3956-1
[33]	Yao YG. 2017. Creating animal models, why not use the Chinese tree shrew (Tupaia belangeri chinensis)?. Zoological Research, 38(3): 118−126. doi: 10.24272/j.issn.2095-8137.2017.032
[34]	Ye LH, He M, Huang YC, Zhao GQ, Lei YJ, Zhou YC, et al. 2016. Tree shrew as a new animal model for the study of lung cancer. Oncology Letters, 11(3): 2091−2095. doi: 10.3892/ol.2016.4156
[35]	Zhang XH, Dai ZX, Zhang GH, Han JB, Zheng YT. 2013. Molecular characterization, balancing selection, and genomic organization of the tree shrew (Tupaia belangeri) MHC class I gene. Gene, 522(2): 147−155. doi: 10.1016/j.gene.2013.03.113
[36]	Zheng LZ, Liu Z, Lei M, Peng J, He YX, Xie XH, et al. 2013. Steroid-associated hip joint collapse in bipedal emus. PLoS One, 8(10): e76797. doi: 10.1371/journal.pone.0076797
[37]	Zheng LZ, Wang JL, Kong L, Huang L, Tian L, Pang QQ, et al. 2018. Steroid-associated osteonecrosis animal model in rats. Journal of Orthopaedic Translation, 13: 13−24. doi: 10.1016/j.jot.2018.01.003