Allele-specific expression and alternative splicing in horse×donkey and cattle×yak hybrids

Yu Wang; Shan Gao; Yue Zhao; Chen Wei-Huang; Jun-Jie Shao; Wang Ni-Ni; Ming Li; Guang-Xian Zhou; Lei Wang; Wen-Jing Shen; Jing-Tao Xu; Wei-Dong Deng; Wen Wang; Yu-Lin Chen; Yu Jiang

doi:10.24272/j.issn.2095-8137.2019.042

Volume 40 Issue 4

Jul. 2019

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Article Navigation > Zoological Research > 2019 > 40(4): 293-305

Yu Wang, Shan Gao, Yue Zhao, Chen Wei-Huang, Jun-Jie Shao, Wang Ni-Ni, Ming Li, Guang-Xian Zhou, Lei Wang, Wen-Jing Shen, Jing-Tao Xu, Wei-Dong Deng, Wen Wang, Yu-Lin Chen, Yu Jiang. Allele-specific expression and alternative splicing in horse×donkey and cattle×yak hybrids. Zoological Research, 2019, 40(4): 293-305. doi: 10.24272/j.issn.2095-8137.2019.042

Citation:

Yu Wang, Shan Gao, Yue Zhao, Chen Wei-Huang, Jun-Jie Shao, Wang Ni-Ni, Ming Li, Guang-Xian Zhou, Lei Wang, Wen-Jing Shen, Jing-Tao Xu, Wei-Dong Deng, Wen Wang, Yu-Lin Chen, Yu Jiang. Allele-specific expression and alternative splicing in horse×donkey and cattle×yak hybrids. Zoological Research, 2019, 40(4): 293-305. doi: 10.24272/j.issn.2095-8137.2019.042

Citation:

Yu Wang, Shan Gao, Yue Zhao, Chen Wei-Huang, Jun-Jie Shao, Wang Ni-Ni, Ming Li, Guang-Xian Zhou, Lei Wang, Wen-Jing Shen, Jing-Tao Xu, Wei-Dong Deng, Wen Wang, Yu-Lin Chen, Yu Jiang. Allele-specific expression and alternative splicing in horse×donkey and cattle×yak hybrids. Zoological Research, 2019, 40(4): 293-305. doi: 10.24272/j.issn.2095-8137.2019.042

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Allele-specific expression and alternative splicing in horse×donkey and cattle×yak hybrids

doi: 10.24272/j.issn.2095-8137.2019.042

1.
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, China
2.
Stake Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining Qinghai 810016, China
3.
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China
4.
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming Yunnan 650223, China

Funds: This work was supported by grants from the National Natural Science Foundation of China (31572381), National Thousand Youth Talents Plan of the International Science and Technology Cooperation Project of China (2013DFA31420), and Science and Technology Innovation Capability Promotion Program of the Science and Technology Department of Qinghai Province (2015-ZJ-712)

More Information

Corresponding author: Yu Jiang
Received Date: 2019-04-01
Publish Date: 2019-07-18

Abstract

Abstract

Divergence of gene expression and alternative splicing is a crucial driving force in the evolution of species; to date, however the molecular mechanism remains unclear. Hybrids of closely related species provide a suitable model to analyze allele-specific expression (ASE) and allele-specific alternative splicing (ASS). Analysis of ASE and ASS can uncover the differences in cis-regulatory elements between closely related species, while eliminating interference of trans-regulatory elements. Here, we provide a detailed characterization of ASE and ASS from 19 and 10 transcriptome datasets across five tissues from reciprocal-cross hybrids of horse×donkey (mule/hinny) and cattle×yak (dzo), respectively. Results showed that 4.8%–8.7% and 10.8%–16.7% of genes exhibited ASE and ASS, respectively. Notably, lncRNAs and pseudogenes were more likely to show ASE than protein-coding genes. In addition, genes showing ASE and ASS in mule/hinny were found to be involved in the regulation of muscle strength, whereas those of dzo were involved in high-altitude adaptation. In conclusion, our study demonstrated that exploration of genes showing ASE and ASS in hybrids of closely related species is feasible for species evolution research.
- Allele-specific alternative splicing,
- Allele-specific expression,
- Cis-regulatory elements

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References(1)

References

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