Volume 38 Issue 2
Mar.  2017
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Shi-Hao Wu, Zhi-Xing Liao, Joshua D. Rizak, Na Zheng, Lin-Heng Zhang, Hen Tang, Xiao-Bin He, Yang Wu, Xia-Ping He, Mei-Feng Yang, Zheng-Hui Li, Dong-Dong Qin, Xin-Tian Hu. Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains. Zoological Research, 2017, 38(2): 88-95. doi: 10.24272/j.issn.2095-8137.2017.015
Citation: Shi-Hao Wu, Zhi-Xing Liao, Joshua D. Rizak, Na Zheng, Lin-Heng Zhang, Hen Tang, Xiao-Bin He, Yang Wu, Xia-Ping He, Mei-Feng Yang, Zheng-Hui Li, Dong-Dong Qin, Xin-Tian Hu. Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains. Zoological Research, 2017, 38(2): 88-95. doi: 10.24272/j.issn.2095-8137.2017.015

Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains

doi: 10.24272/j.issn.2095-8137.2017.015
Funds:  This study was supported by the National Program on Key Basic Research Project (973 Programs 2015CB755605) and the National Natural Science Foundation of China (81471312)
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  • Corresponding author: Dong-Dong Qin, Xin-Tian Hu
  • Received Date: 2017-01-09
  • Rev Recd Date: 2017-03-03
  • Publish Date: 2017-03-18
  • Viral vector transfection systems are among the simplest of biological agents with the ability to transfer genes into the central nervous system. In brain research, a series of powerful and novel gene editing technologies are based on these systems. Although many viral vectors are used in rodents, their full application has been limited in non-human primates. To identify viral vectors that can stably and effectively express exogenous genes within non-human primates, eleven commonly used recombinant adeno-associated viral and lentiviral vectors, each carrying a gene to express green or red fluorescence, were injected into the parietal cortex of four rhesus monkeys. The expression of fluorescent cells was used to quantify transfection efficiency. Histological results revealed that recombinant adeno-associated viral vectors, especially the serotype 2/9 coupled with the cytomegalovirus, human synapsin I, or Ca2+/calmodulin-dependent protein kinase II promoters, and lentiviral vector coupled with the human ubiquitin C promoter, induced higher expression of fluorescent cells, representing high transfection efficiency. This is the first comparison of transfection efficiencies of different viral vectors carrying different promoters and serotypes in non-human primates (NHPs). These results can be used as an aid to select optimal vectors to transfer exogenous genes into the central nervous system of non-human primates.
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