Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (<em>Macaca leonina</em>)

Xiao-Liang ZHANG; Jia-Hao SONG; Wei PANG; Yong-Tang ZHENG

doi:10.13918/j.issn.2095-8137.2016.4.246

Volume 37 Issue 4

Jul. 2016

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Xiao-Liang ZHANG, Jia-Hao SONG, Wei PANG, Yong-Tang ZHENG. Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina). Zoological Research, 2016, 37(4): 246-251. doi: 10.13918/j.issn.2095-8137.2016.4.246

Citation:

Xiao-Liang ZHANG, Jia-Hao SONG, Wei PANG, Yong-Tang ZHENG. Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina). Zoological Research, 2016, 37(4): 246-251. doi: 10.13918/j.issn.2095-8137.2016.4.246

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Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina)

doi: 10.13918/j.issn.2095-8137.2016.4.246

1 Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;
2 Institute of Health Sciences, Anhui University, Hefei Anhui 230601, China;
3 Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China;
4 Kunming Primate Research Center of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China

Funds: This work was supported by the National Special Science Research Program of China (2012CBA01305), National Natural Science Foundation of China (81172876; 81471620; 81273251; 81571606; U0832601), National Science and Technology Major Project (2014ZX10005-002-006), Knowledge Innovation Program of CAS (KJZD-EW-L10-02) and Yunnan Applicative and Basic Research Program (2014FB181)

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Corresponding author: Xiao-Liang ZHANG
Received Date: 2016-05-23
Rev Recd Date: 2016-07-05
Publish Date: 2016-07-18

Abstract

Abstract

Northern pig-tailed macaques (NPMs, Macaca leonina) are susceptible to HIV-1 infection largely due to the loss of HIV-1-restricting factor TRIM5α. However, great impediments still exist in the persistent replication of HIV-1 in vivo, suggesting some viral restriction factors are reserved in this host. The APOBEC3 proteins have demonstrated a capacity to restrict HIV-1 replication, but their inhibitory effects in NPMs remain elusive. In this study, we cloned the NPM A3A-A3H genes, and determined by BLAST searching that their coding sequences (CDSs) showed 99% identity to the corresponding counterparts from rhesus and southern pig-tailed macaques. We further analyzed the anti-HIV-1 activities of the A3A-A3H genes, and found that A3G and A3F had the greatest anti-HIV-1 activity compared with that of other members. The results of this study indicate that A3G and A3F might play critical roles in limiting HIV-1 replication in NPMs in vivo. Furthermore, this research provides valuable information for the optimization of monkey models of HIV-1 infection.
- Macaca leonina,
- Northern pig-tailed macaques,
- APOBEC3

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References(33)

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