Volume 37 Issue 4
Jul.  2016
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Li-Fang JIN, Jin-Song LI. Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems. Zoological Research, 2016, 37(4): 205-213. doi: 10.13918/j.issn.2095-8137.2016.4.205
Citation: Li-Fang JIN, Jin-Song LI. Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems. Zoological Research, 2016, 37(4): 205-213. doi: 10.13918/j.issn.2095-8137.2016.4.205

Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems

doi: 10.13918/j.issn.2095-8137.2016.4.205
Funds:  This study was supported by the National Natural Science Foundation of China (3731530048C1202)
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  • Corresponding author: Li-Fang JIN
  • Received Date: 2016-04-11
  • Rev Recd Date: 2016-05-05
  • Publish Date: 2016-07-18
  • With the development of high-throughput sequencing technology in the post-genomic era, researchers have concentrated their efforts on elucidating the relationships between genes and their corresponding functions. Recently, important progress has been achieved in the generation of genetically modified mice based on CRISPR/Cas9 and haploid embryonic stem cell (haESC) approaches, which provide new platforms for gene function analysis, human disease modeling, and gene therapy. Here, we review the CRISPR/Cas9 and haESC technology for the generation of genetically modified mice and discuss the key challenges in the application of these approaches.
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