Volume 35 Issue 3
May  2014
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Xiao-Liang ZHANG, Wei PANG, De-Yao DENG, Long-Bao LV, Yue FENG, Yong-Tang ZHENG. Analysis of immunoglobulin, complements and CRP levels in serum of captive northern pig-tailed macaques (Macaca leonina). Zoological Research, 2014, 35(3): 196-203. doi: 10.11813/j.issn.0254-5853.2014.3.196
Citation: Xiao-Liang ZHANG, Wei PANG, De-Yao DENG, Long-Bao LV, Yue FENG, Yong-Tang ZHENG. Analysis of immunoglobulin, complements and CRP levels in serum of captive northern pig-tailed macaques (Macaca leonina). Zoological Research, 2014, 35(3): 196-203. doi: 10.11813/j.issn.0254-5853.2014.3.196

Analysis of immunoglobulin, complements and CRP levels in serum of captive northern pig-tailed macaques (Macaca leonina)

doi: 10.11813/j.issn.0254-5853.2014.3.196
  • Received Date: 2013-09-03
  • Rev Recd Date: 2013-12-01
  • Publish Date: 2014-05-08
  • The northern pig-tailed macaque (NPM,Macaca leonina) has become a widely used animal model in biomedical research. In this study, we measured serum immunoglobulin IgG, IgM, IgA, complement C3, C4 and CRP levels in 3-11 year old captive northern pig-tailed macaques using HITACHI 7600-20 automated chemistry analyzer in order to determine the influences of age and gender on these items. The results showed that serum IgA, IgM, C3 and C4 levels were not correlated with age (P>0.05), while serum IgG levels increased progressively with age (r=0.202;P=0.045). Serum IgG, IgA, IgM and C3 levels were higher in females than in males (P<0.05). Moreover, serum C3 concentration was both positively and strongly correlated with that of C4 (r=0.700; P<0.0001). This study provides basic serum immunoglobulin and complement data of captive northern pig-tailed macaques, which may prove useful for future breeding efforts and biomedical research.
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  • [1]
    Ahmed SA, Penhale W, Talal N. 1985. Sex hormones, immune responses, and autoimmune diseases. Mechanisms of sex hormone action. The American Journal of Pathology, 121(3): 531-551.
    Ahmed SA, Talal N. 1990. Sex hormones and the immune system-part 2. Animal data. Baillieres Clinical Rheumatology, 4(1): 13-31.
    Albert CM, Ma J, Rifai N, Stampfer MJ, Ridker PM. 2002. Prospective study of C-reactive protein, homocysteine, and plasma lipid levels as predictors of sudden cardiac death. Circulation, 105(22): 2595-2599.
    Allansmith M, Mcclellan B, Butterworth M, Maloney J. 1968. The development of immunoglobulin levels in man. The Journal of Pediatrics, 72(2): 276-290.
    Angstwurm MW, Gärtner R, Ziegler-Heitbrock H. 1997. Cyclic plasma IL-6 levels during normal menstrual cycle. Cytokine, 9(5): 370-374.
    Axberg I, Gale Jr MJ, Afar B, Clark EA. 1991. Characterization of T-cell subsets and T-cell receptor subgroups in pigtailed macaques using two-and three-color flow cytometry. Journal of Clinical Immunology, 11(4): 193-204.
    Cannon JG, Dinarello CA. 1985. Increased plasma interleukin-1 activity in women after ovulation. Science, 227(4691): 1247-1249.
    Chen M, Daha MR, Kallenberg CG. 2010. The complement system in systemic autoimmune disease. Journal of Autoimmunity, 34(3): 276-286.
    Chen Y, Li CH, Tong PF, Sun XM. 2006. Analyze on immunoglobulin IgG, IgM, IgA, Complement C3 and C4 in laboratory rhesus monkey (macaca mulatta) of different ages. Chinese Journal of Comparative Medicine, 16(5): 257-259. (in Chinese)
    Cheng SJ, Huang R, Huang ZY, Yu B. 2003. Antibody and complement levels in serum of rhesus monkey. Chinese Journal of Veterinary Medicine, 39(4): 14-15. (in Chinese)
    Cole MF, Bowen WH. 1976. Immunoglobulins A, G, and M in serum and in some secretions of monkeys (Macaca fascicularis syn. irus). Infection and Immunity, 13(5): 1354-1359.
    Curry BB. 2001. Animal models used in identifying gender-related differences. International Journal of Toxicology, 20(3): 153-160.
    Du Clos TW, Mold C. 2004. C-reactive protein. Immunology Research, 30(3): 261-277.
    Ellingsworth LR, Holmberg CA, Osburn BI. 1983. Hemolytic complement measurement in eleven species of nonhuman primates. Veterinary Immunology and Immunopathology, 5(2): 141-149.
    Falus A, Rokita H, Walcz E, Brozik M, Hidvegi T, Merétey K. 1990. Hormonal regulation of complement biosynthesis in human cell lines. II. Upregulation of the biosynthesis of complement components C3, factor B and Cl inhibitor by interleukin-6 and interleukin-1 in human hepatoma cell line. Molecular Immunology, 27(2): 197-201.
    Fireman P, Zuchowski DA, Taylor PM. 1969. Development of human complement system. The Journal of Immunology, 103(1): 25-31.
    Flores BM, Fennell CL, Kuller L, Bronsdon MA, Morton WR, Stamm WE. 1990. Experimental infection of pig-tailed macaques (Macaca nemestrina) with Campylobacter cinaedi and Campylobacter fennelliae. Infection and Immunology, 58(12): 3947-3953.
    Fujimoto K, Terao K, Cho F, Nakamura F, Honjo S. 1982. Age-related immunoglobulin levels in cynomolgus monkeys. Japanese Journal of Medical Science and Biology, 35(1): 17-23.
    Gewurz H, Pickering RJ, Christian CL, Snyderman R, Mergenhagen SE, Good RA. 1968. Decreased C'1q protein concentration and agglutinating activity in agammaglobulinaemia syndromes: an inborn error reflected in the complement system. Clinical and Experimental Immunology, 3(5): 437-445.
    Grossman CJ. 1984. Regulation of the immune system by sex steroids. Endocrine Reviews, 5(3): 435-455.
    Groves CP. 2001. Primate Taxonomy. Washington DC: Smithsonian Institution Press, 222-224.
    Jinbo T, Hayashi S, Iguchi K, Shimizu M, Matsumoto T, Naiki M, Yamamoto S. 1998. Development of monkey C-reactive protein (CRP) assay methods. Veterinary Immunology and Immunopathology, 61(2-4): 195-202.
    Kasperska-Zajac A, Grzanka A, Machura E, Misiolek M, Mazur B, Jochem J. 2013. Increased serum complement C3 and C4 concentrations and their relation to severity of chronic spontaneous urticaria and CRP concentration. Journal of Inflammation, 10(1): 1-5.
    Khera A, Mcguire DK, Murphy SA, Stanek HG, Das SR, Vongpatanasin W, Wians FH, Grundy SM, De Lemos JA. 2005. Race and gender differences in C-reactive protein levels. Journal of American College of Cardiology, 46(3): 464-469.
    Knapp LA, Ha JC, Sackett GP. 1996. Parental MHC antigen sharing and pregnancy wastage in captive pigtailed macaques. Journal of Reproductive Immunology, 32(1): 73-88.
    Kohler PF, Muller-Eberhard HJ. 1969. Complement-immunoglobulin relation: Deficiency of C'lq associated with impaired immunoglobulin G synthesis. Science, 163(3866): 474-475.
    Kuang YQ, Tang X, Liu FL, Jiang XL, Zhang YP, Gao GX, Zheng YT. 2009. Genotyping of TRIM5 locus in Northern pig-tailed macaques (Macaca leonina), a primate species susceptible to human immunodeficiency virus type 1 infection. Retrovirology, 6(1): 58.
    Lei A, Pang W, Zhang GH, Zheng YT. 2013. Use and research of pigtailed macaques in nonhuman primate HIV/AIDS models. Zoological Research, 34(2): 77-88. (in Chinese)
    Li X, Polacino P, Garcia-Navarro R, Hu SL, Tsuji M. 2012. Peripheral blood invariant natural killer T cells of pig-tailed macaques. PLoS One, 7(10): e48166.
    Macellini S, Ferrari PF, Bonini L, Fogassi L, Paukner A. 2010. A modified mark test for own-body recognition in pig-tailed macaques (Macaca nemestrina). Animal Cognition, 13(4): 631-639.
    Maes M, Hendriks D, Van Gastel A, Demedts P, Wauters A, Neels H, Janca A, Scharpé S. 1997. Effects of psychological stress on serum immunoglobulin, complement and acute phase protein concentrations in normal volunteers. Psychoneuroendocrinology, 22(6): 397-409.
    Mayer P, Geissler K, Ward M, Metcalf D. 1993. Recombinant human leukemia inhibitory factor induces acute phase proteins and raises the blood platelet counts in nonhuman primates. Blood, 81(12): 3226-3233.
    Miller C, Kang D, Marthas M, Moldoveanu Z, Kiyono H, Marx P, Eldridge J, Mestecky J, Mcghee J. 1992. Genital secretory immune response to chronic simian immunodeficiency virus (SIV) infection: a comparison between intravenously and genitally inoculated rhesus macaques. Clinical and Experimental Immunology, 88(3): 520-526.
    Morales JC, Melnick DJ. 1998. Phylogenetic relationships of the macaques (Cercopithecidae: Macaca), as revealed by high resolution restriction site mapping of mitochondrial ribosomal genes. Journal of Human Evolution, 34(1): 1-23.
    Pang W, Lü LB, Wang Y, Li G, Huang DT, Lei AH, Zhang GH, Zheng YT. 2013. Measurement and analysis of hematology and blood chemistry parameters in northern pig-tailed macaques (Macaca leonina). Zoological Research, 34(2): 89-96. (in Chinese)
    Patterson R, Harris K, Suszko I, Roberts M. 1976. Reagin-mediated asthma in rhesus monkeys and relation to bronchial cell histamine release and airway reactivity to carbocholine. The Journal of Clinlinical Investigation, 57(3): 586-593.
    Patton DL, Cosgrove-Sweeney YT, Rabe LK, Hillier SL. 2001. The pig-tailed macaque rectal model: microflora and chlamydial infection. Sexually Transmitted Diseases, 28(7): 363-366.
    Plebani A, Notarangelo L, Duse M, Avanzini A, Massa M, Ugazio A. 1984. Serum IgG levels and complement activity in hypogammaglobulinaemic patients under substitution therapy. Clinical Experimental Immunology, 58(1): 193-198.
    Poskitt TR, Fortwengler Jr HP, Bobrow JC, Roth GJ. 1974. Naturally occurring immune-complex glomerulonephritis in monkeys (Macaca irus): I. Light, immunofluorescence and electron microscopic studies. The American Journal of Pathology, 76(1): 145-164.
    Rausell E, Bickford L, Manger PR, Woods TM, Jones EG. 1998. Extensive divergence and convergence in the thalamocortical projection to monkey somatosensory cortex. The Journal of Neuroscience, 18(11): 4216-4232.
    Ritchie RF, Palomaki GE, Neveux LM, Navolotskaia O, Ledue TB, Craig WY. 2004. Reference distributions for complement proteins C3 and C4: a practical, simple and clinically relevant approach in a large cohort. Journal of Clinical Laboratory Analysis, 18(1): 1-8.
    Roach B, Kim Y, Jerome E, Michael AF. 1981. Influence of age and sex on serum complement components in children. Archives of Pediatrics and Adolescent Medicine, 135(10): 918-920.
    Saadi S, Takahashi T, Holzknecht RA, Platt JL. 2004. Pathways to acute humoral rejection. The American Journal of Pathology, 164(3): 1073-1080.
    Shaulov A, Murali-Krishna K. 2008. CD8 T cell expansion and memory differentiation are facilitated by simultaneous and sustained exposure to antigenic and inflammatory milieu. The Journal of Immunology, 180(2): 1131-1138.
    Shuster J, Warner NL, Fudenberg HH. 1969. Discussion paper: cross-reactivity of primate immunoglobulins. Annals of the New York Academy of Sciences, 162(1): 195-201.
    Socha W, Blancher A, Ruffie J. 1993. Comparative study of human monoclonal anti-D antibodies of IgG and IgM classes in tests with red cells of nonhuman primates. Revue Française de Transfusion et d'Hémobiologie, 36(6): 485-497.
    Sussman A, Ha J. 2011. Developmental and cross-situational stability in infant pigtailed macaque temperament. Developmental Psychology, 47(3): 781-791.
    Verthelyi D, Klinman D. 2000. Sex hormone levels correlate with the activity of cytokine‐secreting cells in vivo. Immunology, 100(3): 384-390.
    Wagner E, Frank MM. 2009. Therapeutic potential of complement modulation. Nature Reviews Drug Discovery, 9(1): 43-56.
    Watts KL, Nelson V, Wood BL, Trobridge GD, Beard BC, Humphries RK, Kiem HP. 2012. Hematopoietic stem cell expansion facilitates multilineage engraftment in a nonhuman primate cord blood transplantation model. Experimental Hematology, 40(3): 187-196.
    Yilmazer M, Fenkci V, Fenkci S, Aktepe O, Sonmezer M, Kurtay G. 2003. Association of serum complement (C3, C4) and immun­oglobulin (IgG, IgM) levels with hormone replacement therapy in healthy post-menopausal women. Human Reproduction, 18(7): 1531-1535.
    Yoshino N, Ami Y, Terao K, Tashiro F, Honda M. 2000. Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Experimental Animal, 49(2): 97-110.
    Yu CY, Whitacre CC. 2004. Sex, MHC and complement C4 in autoimmune diseases. Trends in Immunology, 25(12): 694-699.
    Zhang XH, Li GT, Zhang ZL. 2011. Clinical significances of C-reactive protein and hypersensitive C-reactive protein. Chinese Journal of Allergy and Clinical Immunology, 5(1):74-79. (in Chinese)
    Zhu L, Zhang GH, Zheng YT. 2010. Application studies of animal models in evaluating safety and efficacy of HIV-1 microbicides. Zoological Research, 31(1): 66-76. (in Chinese)
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