Volume 35 Issue 2
Mar.  2014
Turn off MathJax
Article Contents
Shou-Jie TANG, Si-Fa LI, Wan-Qi CAI, Yan ZHAO. Microsatellite analysis of variation among wild, domesticated, and genetically improved populations of blunt snout bream (Megalobrama amblycephala). Zoological Research, 2014, 35(2): 108-117. doi: 10.11813/j.issn.0254-5853.2014.2.108
Citation: Shou-Jie TANG, Si-Fa LI, Wan-Qi CAI, Yan ZHAO. Microsatellite analysis of variation among wild, domesticated, and genetically improved populations of blunt snout bream (Megalobrama amblycephala). Zoological Research, 2014, 35(2): 108-117. doi: 10.11813/j.issn.0254-5853.2014.2.108

Microsatellite analysis of variation among wild, domesticated, and genetically improved populations of blunt snout bream (Megalobrama amblycephala)

doi: 10.11813/j.issn.0254-5853.2014.2.108
  • Received Date: 2013-09-18
  • Rev Recd Date: 2013-12-11
  • Publish Date: 2014-03-08
  • In the present study, the genetic diversity of one selected strain (Pujiang No. 1), two domesticated populations (GA and HX) and four wild populations (LZ, YN, SS and JL) of blunt snout bream (Megalobrama amblycephala) was analyzed using 17 microsatellite markers. The results showed that an average of 4.88-7.65 number of alleles (A); an average of 3.20-5.33 effective alleles (Ne); average observed heterozygosity (Ho) of 0.6985-0.9044; average expected heterozygosity (He) of 0.6501-0.7805; and the average polymorphism information content (PIC) at 0.5706-0.7226. Pairwise FST value between populations ranged from 0.0307-0.1451, and Nei's standard genetic distance between populations was 0.0938-0.4524. The expected heterozygosities in the domesticated populations (GA and HX) were significantly lower than those found in three wild populations (LZ, SS and JL), but no difference was detected when compared with the wild YN population. Likewise, no difference was found between the four wild populations or two domesticated populations. The expected heterozygosity in Pujiang No. 1 was higher than the two domesticated populations and lower than the four wild populations. Regarding pairwise FST value between populations, permutation test P-values were significant between the GA, HX and PJ populations, but not between the four wild populations. These results showed that the expected heterozygosity in the selected strain of blunt snout bream, after seven generations of selective breeding, was lower than that of wild populations, but this strain retains higher levels of genetic diversity than domesticated populations. The genetic differences and differentiation amongst wild populations, domesticated populations and the genetically improved strain of blunt snout bream will provide important conservation criteria and guide the utilization of germplasm resources.
  • loading
  • [1]
    Aung O, Nguyen TTT, Poompuang S, Kamonrat W. 2010. Microsatellite DNA markers revealed genetic population structure among captive stocks and wild populations of mrigal, Cirrhinus cirrhosus in Myanmar. Aquaculture, 299(1-4): 37-43.
    Bian CY, Dong S, Tan SZ. 2007. PCR - RFLP analysis of mtDNA D-loop region of three populations of blunt nose black bream Megalobrama amblycephala. Journal of Dalian Fisheries University, 22(3): 175-179. (in Chinese with English abstract)
    Botstein D, White RL, Skolnick M, Davis RW. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3): 314-331.
    Caughley G. 1994. Directions in conservation biology. The Journal of Animal Ecology, 63(2): 215-244.
    Clifford SL, Meginnity P, Feguson A. 1998. Genetic changes in an Atlantic salmon population resulting from escaped juvenile farm salmon. Journal of Fish Biology, 52(1): 118-127.
    Coughlan JP, Imsland AK, Galvin PT, Fitzgerald RD, Naevdal G, Cross TF. 1998. Microsatellite DNA variation in wild populations and farmed strains of turbot from Ireland and Norway: a preliminary study. Journal of Fish Biology, 52(5): 916-922.
    Desvignes JF, Laroche J, Durand JD, Bouvet Y. 2001. Genetic variability in reared stocks of common carp (Cyprinus carpio L.) based on allozymes and microsatellites. Aquaculture, 194(3-4): 291-301.
    Eknath AE, Doyle RW. 1985. Indirect selection for growth and life-history traits in Indian carp aquaculture: I. Effects of broodstock management. Aquaculture, 49(1): 73-84.
    Excoffier L, Laval G, Schneider S. 2005. Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1: 47-50.
    Ke HW. 1965. The artificial reproduction and culture experiment of Megalobrama amblycephala. Acta Hydrobiologica Sinica, 5: 282-283. (in Chinese with English abstract)
    Kumar S, Tamura K, Nei M. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics, 5(2): 150-163.
    Leberg PL. 1992. Effects of population bottlenecks on genetic diversity as measured by allozyme electrophoresis. Evolution, 46(2): 471-494.
    Li Q, Park C, Endo T, Kijima A. 2004. Loss of genetic variation at microsatellite loci in hatchery strains of the Pacific abalone (Haliotis discus hannai). Aquaculture, 235(1-4): 207-222.
    Li SF. 1988. Conservation of the genetic performance of fish breeding populations. Journal of Fisheries of China, 12(3): 283-290. (in Chinese with English abstract)
    Li SF, Yang XM. 1996. Effects of two-way selection on biochemical genetics of blunt snout bream (Megalobrama amblycephala). Journal of Fishery Sciences of China, 3(1): 1-5. (in Chinese with English abstract)
    Li SF, Cai WQ. 2000. Two-way selective response of Megalobrama amblycephala. Journal of Fisheries of China, 24(3): 201-205. (in Chinese with English abstract)
    Li SF, Cai WQ. 2003. Genetic improvement of the herbivorous blunt snout bream (Megalobrama amblycephala). NAGA, 26(1): 20-23.
    Li SF, Cai WQ, Zhou BY. 1991a. Morphological and biochemical genetic variations among populations of blunt snout bream (Megalobrama amblycephala). Journal of Fisheries of China, 15(3): 204-211. (in Chinese with English abstract)
    Li SF, Zhou BY, Ling GQ. 1991b. The growth and reproduction of Blunt snout bream in Yuni Lake. Chinese Journal of Zoology, 26(6): 7-12, 22. (in Chinese with English abstract)
    Li SF, Yang HY, Zou SM. 2005. Effects on genetic structure from quick inbreeding and estimation of inbreeding response in cultured populations of Megalobrama amblycephala. Journal of Fisheries of China, 29(2): 161-165. (in Chinese with English abstract)
    Li SW, Chang YM, Liang LQ, Sun XW. 2006. Rapid isolation of microsatellites from genome of Bluntnose black bream (Megalobrama amblycephala). Journal of Fishery Sciences of China, 13(2): 187-192. (in Chinese with English abstract)
    Li WT, Liao XL, Yu XM, Wang D, Tong J. 2007. Isolation and characterization of polymorphic microsatellite loci in Wuchang bream (Megalobrama amblycephala). Molecular Ecology Notes, 7(5): 771-773.
    Liu B, Kuang YY, Tong GX, Yin JS. 2011. Analysis of genetic diversity on 9 wild stocks of Taimen (Hucho taimen) by microsatellite markers. Zoological Research, 32(6): 597-604. (in Chinese with English abstract)
    Nei M. 1972. Genetic distance between populations. The American Naturalist, 106(949): 283-292.
    Nei M. 1987. Molecular Evolutionary Genetics. New York: Columbia University Press.
    Nei M, Maruyama T, Chakraborty R. 1975. The bottleneck effect and genetic variability in populations. Evolution, 29(1): 1-10.
    Nei M, Tajima F, Tateno Y. 1983. Accuracy of estimated phylogenetic trees from molecular data. Journal of Molecular Evolution, 19(2): 153-170.
    Norris AT, Bradley DG, Cunningham EP. 1999. Microsatellite genetic variation between and within farmed and wild Atlantic salmon (Salmo salar) populations. Aquaculture, 180(3-4): 247-264.
    Ota T. 1993. DISPAN: Genetic Distance and Phylogenetic Analysis Software. Pennsylvania State University, Pennsylvania. http://oat.bio. indiana.edu: 7580/documents/public/molbio/genetic/pop/Dispan/.
    Saitou N, Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4(4): 406-425.
    Sambrook J, Russell DW. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. New York: Cold Spring Harbor Laboratory Press.
    Šegvić-Bubić T, Lepen I, Trumbić Z, Ljubković J, Sutlović D, Matić-Skoko S, Grubišić L, Glamuzina B, Mladineo I. 2011. Population genetic structure of reared and wild gilthead sea bream (Sparus aurata) in the Adriatic Sea inferred with microsatellite loci. Aquaculture, 318(3-4): 309-315.
    Sokal RR, Rohlf FJ. 1995. Biometry: Principles and Practice of Statistics in Biological Research. 3rd ed. New York: W. H. Freeman and Co..
    Takezaki N, Nei M. 1996. Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genetics, 144(1): 389-399.
    Tang SJ, Li SF, Cai WQ. 2009. Development of microsatellite markers for blunt snout bream Megalobrama amblycephala using 5′-anchored PCR. Molecular Ecology Resources, 9(3): 971-974.
    Utter F, Hindar K, Ryman N. 1993. Genetic effects of aquaculture on natural salmonid populations. In: Heen, K, Monahan RL, Utter F. Salmon Aquacultur. Oxford: Fishing News Books, 144-165.
    Wachirachaikarn A, Prakoon W, Nguyen TTT, Prompakdee W, Na-Nakorn U. 2011. Loss of genetic variation of Phalacronotus bleekeri (Günther, 1864) in the hatchery stocks revealed by newly developed microsatellites. Aquaculture, 321(3-4): 298-302.
    Weir BS, Cockerham CC. 1984. Estimating F-statistics for the analysis of population structure. Evolution, 38(6): 1358-1370.
    Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX. 1997. POPGENE, the User-Friendly Shareware for Population Genetic Analysis. Canada: Molecular Biology and Biotechnology Centre, University of Alberta.
    Yi BL. 1955. Notes on Megalobrama amblycephala, sp. nov., a distinct species from M. terminalis (Richardson). Acta Hydrobiologica Sinica, (2): 115-122. (in Chinese with English abstract)
    Zhang DC. 2001. Study on genetic diversity of bluntnose black bream from Yunihu and Liangzihu Lakes. Journal of China Three Gorges University: Natural Sciences, 23(3): 282-284. (in Chinese with English abstract)
    Zhang ZW, Cao ZM, Yang H, Wang JL, Cao JL, Han YP, Wu TT. 2006. Microsatellites analysis on genetic variation between wild and cultured populations of Ctenopharyngodon idella. Zoological Research, 27(2): 189-196. (in Chinese with English abstract)
    Zhao Y, Li SF, Tang SJ. 2009. Genetic variations among late selected strains and wild populations of blunt snout bream (Megalobrama amblycephala) by ISSR analysis. Journal of Fisheries of China, 33(6): 893-900. (in Chinese with English abstract)
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1489) PDF downloads(1229) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint