Volume 35 Issue 2
Mar.  2014
Turn off MathJax
Article Contents
Jin-Hua WANG, Liang MIAO, Ming-Yun LI, Xiao-Fei GUO, Na PAN, Ying-Ying CHEN, Liang ZHAO. Cloning the Dmrt1 and DmrtA2 genes of ayu (Plecoglossus altivelis) and mapping their expression in adult, larval, and embryonic stages. Zoological Research, 2014, 35(2): 99-107. doi: 10.11813/j.issn.0254-5853.2014.2.099
Citation: Jin-Hua WANG, Liang MIAO, Ming-Yun LI, Xiao-Fei GUO, Na PAN, Ying-Ying CHEN, Liang ZHAO. Cloning the Dmrt1 and DmrtA2 genes of ayu (Plecoglossus altivelis) and mapping their expression in adult, larval, and embryonic stages. Zoological Research, 2014, 35(2): 99-107. doi: 10.11813/j.issn.0254-5853.2014.2.099

Cloning the Dmrt1 and DmrtA2 genes of ayu (Plecoglossus altivelis) and mapping their expression in adult, larval, and embryonic stages

doi: 10.11813/j.issn.0254-5853.2014.2.099
  • Received Date: 2013-08-07
  • Rev Recd Date: 2013-12-16
  • Publish Date: 2014-03-08
  • The Dmrt family of genes are involved in sex differentiation in different species of invertebrates, and some vertebrates including human. In this study, we cloned the full-length cDNA of ayu (Plecoglossus altivelis) Dmrt1 and DmrtA2. Sequence and phylogenetic tree analyses showed ayu Dmrt1 showed highest similarity to that of Oncorhynchus mykiss while ayu DmrtA2 is most similar to that of Oryzias latipes. Fluorescence-based quantitative reverse transcription PCR (qRT-PCR) revealed the Dmrt1 was predominantly expressed in the testis. At the larval stages, Dmrt1 mRNA expression level was highest during 52–64 days post hatching (dph) and at the gastrula stage during embryonic development. DmrtA2, meanwhile, was specifically expressed in the ovary and was highly expressed in the female brain tissue, but not male brain tissue. During the larval stages, DmrtA2 expression remained high before day 34, and then fluctuated while generally decreasing. During embryonic development, DmrtA2 expression increased gradually and peaked at the hatching stage. Our data suggest that ayu Dmrt1 might participate in the differentiation and maintenance of testis while DmrtA2 may play a role in ovary-differentiation and mature-ovary maintenance. DmrtA2 might also participate in brain development.
  • loading
  • [1]
    Cao KJ, Li MY. 1981. The preliminary study of ayu artificial reproduction. Fisheries Science & Technology Information, 9(5): 13-15.
    Cao KJ, Li MY. 1982. Studies on the reproductive biology of the ayu in Fuxi stream, Zhejiang. Journal of Fisheries of China, 6(2): 107-117.
    Capriglione T, Vaccaro MC, Morescalchi MA, Tammaro S, De Iorio S. 2010. Differential DMRT1 expression in the gonads of Podarcis sicula (Reptilia: Lacertidae). Sexual Development, 4(1-2): 104-109.
    de Grandi A, Calvan V, Bertini V, Bulfone A, Peverali G, Camerino G, Borsani G, Guioli S. 2000. The expression pattern of a mouse double sex-related gene is consistent with a role in gonadal differentiation. Mechanisms of Development, 90(2): 323-326.
    Dong S, Nobuhiko T. 2003. Identification of clonal Plecoglossus altivelis using microsatellite DNA marker. Journal of Fisheries of China, 27(4): 295-299.
    Gennet N, Gale E, Nan XS, Farley E, Takacs K, Oberwallner B, Chambers D, Li M. 2011. Doublesex and mab-3-related transcription factor 5 promotes midbrain dopaminergic identity in pluripotent stem cells by enforcing a ventral-medial progenitor fate. Proceedings of the National Academy of Sciences of the United States of America, 108(22): 9131-9136.
    Guan GJ, Kobayashi T, Nagahama Y. 2000. Sexually dimorphic expression of two types of DM (Doublesex/Mab-3)-domain genes in a teleost fish, the tilapia (Oreochromis niloticus). Biochemical and Biophysical Research Communications, 272(3): 662-666.
    Guo YQ, Cheng HH, Huang X, Gao S, Yu HS, Zhou RJ. 2005. Gene structure, multiple alternative splicing, and expression in gonads of zebrafish Dmrt1. Biochemical and Biophysical Research Communications, 330(3): 950-957.
    Guo YQ, Li Q, Gao S, Zhou X, He Y, Shang X, Cheng HH, Zhou RJ. 2004. Molecular cloning, characterization, and expression in brain and gonad of Dmrt5 of zebrafish. Biochemical and Biophysical Research Communications, 324(2): 569-575.
    Huang FY, Li MY. 2004. Biochemical genetic analysis of isozymes in Plecoglossus altivelis population in Fuxi. Journal of Fisheries of China, 28(5): 579-584.
    Kettlewell JR, Raymond CS, Zarkower D. 2000. Temperature-dependent expression of turtle Dmrt1 prior to sexual differentiation. Genesis, 26(3): 174-178.
    Kim S, Kettlewell JR, Anderson RC, Bardwell VJ, Zarkower D. 2003. Sexually dimorphic expression of multiple doublesex-related genes in the embryonic mouse gonad. Gene expression Patterns, 3(1): 77-82.
    Kobayashi T, Matsudua M, Kajiura-Kobayashi H, Suzuki A, Saito N, Nakamoto M, Shibata N, Nagahama Y. 2004. Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes. Developmental Dynamics, 231(3): 518-526.
    Kobayashi T, Kajiura-Kobayashi H, Guan GJ, Nagahama Y. 2008. Sexual dimorphic expression of DMRT1 and SOX9a during gonadal differentiation and hormone-induced sex reversal in the teleost fish Nile tilapia (Oreochromis niloticus). Developmental Dynamics, 237(1): 297-306.
    Kondo M, Froschauer A, Kitano A, Nanda I, Hornung U, Volff JN, Asakawa S, Mitani H, Naruse K, Tanaka M, Schmid M, Shimizu N, Schartl M, Shima A. 2002. Molecular cloning and characterization of DMRT genes from the medaka Oryzias latipes and the platyfish Xiphophorus maculatus. Gnen, 295(2): 213-222.
    Li L, Liang HW, Li Z, Luo XZ, Zhang ZW, Zhu YY, Zou GW. 2012. Cloning and expression analysis of DMRT1 gene in Pelteobagrus fulvidraco. Journal of Huazhong Agricultural University, 31(2): 220-226.
    Li MY, Cao KJ, Xu SL. 1985. A study on the development of the egg, larva and young fish of ayu (Plecoglossus altivelis T.ET S.). Journal of Zhejiang College of Fisheries, 4(1): 35-44.
    Liu XS, Liang B, Zhang SY. 2004. cDNA cloning, tissue distribution and mRNA transcription of DMRT1 gene in the Protandrous Black Porgy Acanthopagrus schlegeli. Zoological Research, 25(2): 158-161.
    Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCT method. Methods, 25(4): 402-408.
    Matson CK, Murphy MW, Sarver AL, Griswold MD, Bardwell VJ, Zarkower D. 2011. DMRT1 prevents female reprogramming in the postnatal mammalian testis. Nature, 476(7358): 101-104.
    Marchand O, Gororoun M, D'Cotta H, McMeel O, Lareyre JJ, Bernot A, Laudet V, Guiguen Y. 2000. DMRT1 expression during gonadal differentiation and spermatogenesis in the rainbow trout, Oncorchynchus mykiss. Biochimica et Biophysica Acta, 1493(1-2): 180-187.
    Nanda I, Shan ZH, Schartl M, Burt DW, Koehler M, Nothwang H, Grützner F, Paton IR, Windsor D, Dunn I, Engel W, Staeheli P, Mizuno S, Haaf T, Schmid M. 1999. 300 million years of conserved synteny between chicken Z and human chromosome 9. Nature Genetics, 21(3): 258-259.
    Ohmuro-Matsuyama Y, Matsuda M, Kobayashi T, Ikeuchi T, Nagahama Y. 2003. Expression of DMY and DMRT1 in various tissues of the Medaka (Oryzias latipes). Zoological Science, 20(11): 1395-1398.
    Parlier D, Moers V, Van Campenhout C, Preillon J, Lecl鑢e L, Saulnier A, Sirakov M, Busengdal H, Kricha S, Marine JC, Rentzsch F, Bellefroid EJ. 2013. The Xenopus doublesex-related gene Dmrt5 is required for olfactory placode neurogenesis. Developmental Biology, 373(1): 39-52.
    Ren LL, Cheng HH, Guo YQ, Huang X, Liu L, Zhou RJ. 2001. Evolutionary conservation of DMRT gene family in amphibians, reptiles and birds. Chinese Science Bulletin, 46(23): 1992-1996.
    Saulnier A, Keruzore M, Clercq SD, Bar I, Moers V, Magnani D, Walcher T, Filippis C, Kricha S, Parlier D, Viviani L, Matson CK, Nakagawa Y, Theil T, Gotz M, Mallamaci A, Marine JC, Zarkower D, Bellefroid EJ. 2013. The doublesex homolog DMRT5 is required for the development of the caudomedial cerebral cortex in mammals. Cerebral Cortex, 23(11): 2553-2567.
    Seo KW, Wang YD, Kokubo H, Kettlewell JR, Zarkower DA, Johnson RL. 2006. Targeted disruption of the DM domain containing transcription factor Dmrt2 reveals an essential role in somite patterning. Developmental Biology, 290(1): 200-210.
    Shen XY, Cui JZ, Yang GP, Gong QL, Gu QQ. 2007. Expression Detection of DMRTs and Two Sox9 Genes in Takifugu rubripes (Tetraodontidae, Vertebrata). Journal of Ocean University of China, 6(2): 182-186.
    Shibata K, Takase M, Nakamura M. 2002. The Dmrt1 expression in sex-reversed gonads of amphibians. General and Comparative Endocrinology, 127(3): 232-241.
    Shin HS, An KW, Park MS, Jeong MH, Choi CY. 2009. Quantitative mRNA expression of sox3 and DMRT1 during sex reversal, and expression profiles after GnRHa administration in black porgy, Acanthopagrus schlegeli. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 154(1): 150-156.
    Smith CA, Roeszler KN, Ohnesorg T, Cummins DM, Farlie PG, Doran TJ, Sinclair AH. 2009. The avian Z-linked gene DMRT1 is required for male sex determination in the chicken. Nature, 461(7261): 267-271.
    Sun YY, Zhang QQ, Qi J, Wang ZG, Chen YJ, Li CM, Zhong QW. 2008. Cloning and expression analysis of DMRT1 gene in Cynoglossus semilaevis. Journal of Wuhan University, 54(2): 221-226.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetic analysis using maximum likelihood, evolutionary distance, and maximum parsimory methods. Molecular Biology and Evolution, 28(10): 2731-2739.
    Veith AM, Schäfer M, Klüver N, Schmidt C, Schultheis C, Schartl M, Winkler C, Volff JN. 2006. Tissue-specific expression of dmrt genes in embryos and adults of the platyfish Xiphophorus maculatus. Zebrafish, 3(3): 325-337.
    Wang GY, Ding WY, Chen SB, Xie QL, Zhou ZM, Ai WM, Wu JG. 2011. RAPD analysis on genetic diversity of four populations of Plecoglossus altivelis from Zhejiang and Fujian province. Bulletin of Science and Technology, 27(6): 863-868.
    Wang HX, Li J, Xiao Y. 1998. The artificial reproduction and embryo development of ayu (Plecoglossus altivelis). Fisheries Science & Technology Information, 25(3): 30-31, 33.
    Yamaguchi A, Lee KH, Fujimoto H, Kadomura K, Yasumoto S, Matsuyama M. 2006. Expression of the DMRT gene and its roles in early gonadal development of the Japanese pufferfish Takifugu rubripes. Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, 1(1): 59-68.
    Yang Y, Gong P, Feng YP, Li SJ, Peng XL, Ran ZP, Qian YG, Gong YZ. 2013. Temporospatial expression of Dmrt1 in chicken urogenital system (Gallus gallus) using whole mount in situ hybridization. Acta Biologica Hungarica, 64(2): 161-168.
    Yu FF, Gui JF, Zhou L, Wang MF, Yu XY. 2009. Cloning and expression characterization of Dmrt5 in Pinctada Martensii. Acta Hydrobiologica Sinica, 2009, 33(5): 844-850.
    Zhang L, Chen B, Cheng HH, Zhou JR. 2006. The cloning and expression analysis of Dmrt5 in rice fieldeel. Hubei Association for Science & Technique. Hubei: Genetic Association of Hubei Province, Genetic Association of Jiangxi, 169.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1304) PDF downloads(2008) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint