A Preliminary Study on the Lamin-like gene of Astasia longa

To investigate whether the unicellular eukaryote euglenoid possess lamin gene,according to known sequences of a series of lower metazoa,a pair of primers were designed to amplify the 3-terminal region of lamin gene.From total DNA of Astasia longa,two fragments were amplified and sequenced.Sequence comparison showed that the short one (650 bp) was the part of 5-terminal of the long one (797 bp).The 797 bp sequence contained a region harboring a motif,"CaaX+stop codon",which is the feature of 3-terminal of all known lamin genes.Our results suggested that the unicellular eukaryote euglenoid possessed a lamin-like gene.