Method for Extracting DNA from Single Rotifer and Sequencing Partical Mitochondria Cytochrome Oxidase Subunit Ⅰ (COⅠ) Gene

Through adjusting doses of regents,experimental conditions and procedure,we extracted DNA from single rotifer using WizardTM genomic DNA purification kit,and sequenced partial COⅠ sequence of Brachionus calyciflorus using the extracted DNA as a template.The obtained sequence shares high similarity with COⅠ sequence of B.plicatilis,showing that it is really a COⅠ sequence of rotifer species,which verified the reliability of our method.