Phylogenetic Analysis of Peritrichs Inferred from Cytosolic Hsp70 Gene Partial Sequences

Cytosolic Hsp70 genes of two peritrichous ciliates (Vorticella campanula & Carchesium polypinum) were partially sequenced and the lengths are 438 bp,both encoding 146 amino acids.Using bacteria as the outgroup,the phylogenetic trees were constructed based on cytosolic Hsp70 amino acid sequences by maximum-likelihood (ML) and neighbor-joining (NJ) methods.The topology that V.campanula clustered with C.polypinum,and then formed a sister clade with another two species in Oligohymenophorea (Tetrahymena thermophila & Paramecium tetraurelia),suggested the monophyly of the peritrichs and their phylogenetic placement in the class Oligohymenophorea.