Cloning and Sequencing of the Sox Genes in Chinemys reevesii

Using a pair of degenerate primers based on the conservative region,HMG-box,of human SRY gene,four different fragments were amplified from both female and male Chinemys reevesii,then cloned by using pGEM-T vector and sequenced.The sequence result indicated their high homology to human Sox genes.The identities to human Sox genes in the DNA sequence and the amino acid sequence are 92%,91%,84%,92% and 100%,93%,98%,98%,respectively.It might be conclud ed that Sox gene was highly conservative in phylogenesis.Our work provides molecular data for the study of sex-determining mechanism of C.reevesii,which might be temperature-dependent sex determination.