Preliminary Investigation on Genetic Diversity of Gynogenetic Silver Crucian Carp (Carassius auratus gibelio Bloch) Detected by Microsatellite DNA

24 individuals from five different gynogenetic strains of silver crucian carp (Carassius auratus gibelio Bloch) were analyzed using 8 microsatellite markers of the poly (CA) type isolated from common carp (Cyprinus carpio L.) by Crooijmans et al.(1997).Electrophoretogram patterns showed that 7 pairs of microsatellite primers reproducibly produced the well-identifiable and homologous DNA fragments except the primers of MFW28.The number of alleles per marker varied from 1 to 14,and the size of alleles ranged from 100 bp to 506 bp.Nearly identical banding patterns occurred among intra-strain individuals,although subtle polymorphism was occasionally detected in the five strains.It was suggested that each strain might possess specific pattern owing to its gynogenesis.On other hand,the five strains differed from each other in electrophoretogram patterns.A total of 23 polymorphic fragments were scored from 5 pairs of primers after excluding bands that were monomorphic for the five strains.The electrophoretogram pattern clearly indicated their intra-strain homogeneity,inter-strain heterogeneity,and their phylogenetic relationships.Strain P is same to strain A,and strain F originates from strain E.Strains A,D and E may originate from different multiple events of hybridization respectively.The microsatellite markers produced by this study will be of benefit to further studies on the evolutionary genetics and provide useful tools for marker-assisted selection breeding or genetic map.