Genetic Diversity of Zhejiang Native Pig Breeds Inferred From Mitochondrial DNA Polymorphism

Restriction fragment length polymorphism (RFLP) of mitochondrial DNA (mtDNA) from Zhejiang native pigs and wild boars was analyzed by using 25 restriction endonucleases,I.e.ApaⅠ,AvaⅠ,Bam HⅠ,BclⅠ,BglⅠ,BglⅡ,ClaⅠ,DraⅠ,Eco RⅠ,Eco R V,Hae Ⅱ,Hin cⅡ,Hin dⅢ,HpaⅠ,KpnⅠ,PstⅠ,PvuⅠ,PvuⅡ,SacⅡ,SalⅠ,Sca Ⅰ,SmaⅠ,StuⅠ,XbaⅠand XhoⅠ,which recognize six nucleotides.In the 30 analyzed individuals,30 restriction morphs,which could be sorted into 3 haplotypes,were detected.Four new morphs,I.e.Bam HⅠ-B,BclⅠ-B,DraⅠ-B and XbaⅠ-C were detected.The average genetic distance (P) was 0.005,and the value of average nucleotide diversity (π) in pig population(including native pigs and wild boars)was 0.0008.This suggested that the genetic diversity of pigs was remarkably scarce.The pigs in Zhejiang might have originated from a late common ancester.Combining with the data from previously reports,it was suggested that the Jinhua strain Ⅲ pigs may have two female origins.