The Regulation of GABA on Development of GABA Receptors in Embryonic Mice Cerebral Cortex

In order to investigate the regulation of GABA on development of GABA receptors in embryonic mice cerebral cortex,GABA and giands were used to treat the embryonic mouse cerebral cortex cells (EMCC) in culture and embryonic mice.GABA receptors were measured by radio giand binding method.The results showed that:1) GABA could increase in the number of GABAA and GABAB receptors of EMCC of 15-17 day embryonic mouse in culture and postnatal day 1(PN 1) mouse cerebral cortex in vivo,and this effect could be mimicked by muscimol and baclofen,and blocked by bicuculline.2) Treated with GABA from embryonic day 7 to 13,the number of GABAA and GABAB receptors of PN 1 mice cerebral cortex remained unchanged.3) Treated with GABA from embryonic day 14 to 19,the number of GABAA receptor of PN 1 mice cerebral cortex increased,but that of GABAB receptor remained unchanged.4) Treated with GABA from embryonic day 7 to 19,the number of GABAA and GABAB receptors of PN 1 mice cerebral cortex increased significantly.The results suggested that GABA could promote the expression of GABA receptors in special stage of embryonic development,and this action was mediated by GABA receptors.