Studies on Nuclear Transplantation of Somatic Cells in Teleost

Nuclei of kidney cells of telecost from different genuses,families and orders were used as materials for nuclear transplantation experiments.The somatic cell nuclei (kidney cell) of crucian (Carassius auratus),mud carp (Cirrhinus molitorella) and mouth breeder (Tilapia nilotica) were transplanted into the enucleated egg cytoplasm of carp (Cyprinus carpio).By means of the serial nuclear transplantation,a nucleocytoplasmic hybrid (NCH) embryo developing into blood circulation stage was obtained in intergenus combination (nucleus of crucian and enucleated egg cytoplasm of carp),a NCH embryo of heart beat stage was obtained in interfamily combination (nucleus of mud carp and enucleated egg cytoplasm of carp),and a NCH embryo of muscular contraction stage was obtained in inter-order combination (nucleus of mouth breeder and enucleated egg cytoplasm of carp).Since these nuclei were taken directly from the living somatic cells of several kinds of fish and then transplanted them into the enucleated eggs of other fish,it was unnecessary,for obtaining synchronization of spawing periods between the donor fish and the recipient fish.The results obtained in this experiment indicated that this method might be useful for making nucleocytoplasmic hybrids in telecost.