Study on phosphomonoesterase,Rnase and dnase in spider (Lycosa singoriensis) Venom

Activities of phosphomonoesterase and nucleases hydrolysing RNA and DNA were presented in a solution of the crude venom from a spider (Lycosa singoriensis) collected in Xinjiang Region,China.The substrates for assaying these enzymes were 4-nitrophenyl phosphate disodium salt,3 terminally 32plabellod RNA and nonlabelled DNA respectively.After incubation of RNA and DNA with the venom solution,the products were detected by polyacrylamide gel or agarose gel electrophoresis.