The application of cell synchronization to chromosome banding of fish and The tentative karyotype analysis of Ctenopharyngodon idellus

Combining cell synchronization in vitro and bromodeoxyuridine (BrdU) incorporation in early synthesis phases and late synthesis phase of cells of Ctenopharyngodon idellus,two kinds of chromosome patterns of replication banding,R- and G-bands were gained after chromosome preparation were irradiated in warm CaCI2 solution by UV and were stained with Giemsa.Some spreads lengthcncd in late-prophase and prometaphase show more numbers of bands and characteristics of higher resolution.Analysis of G-banded karyotype showe that there are four pairs of chromosomes with satellite.A tentative idiogrammatic representation of G-banded karyotype of Gtenopharyngodon idellusis proposed.The significance,techniques and possible meehanism of chromosome banding of fish are discussed.