A new micro-spreading Technique for Demonstrating synaptonemal Complexes in Mammalian spermatocytes

A new micro-spreading technique for demonstrating synaptonemal complexes (SC) has been described.This method gives more consistent results than the routine spreading technique.Furthermore,the ease and speed should allow succssful application of this new simple method to field cytogenetic work.In addition,our results also indicated that both fresh and long-time frozen spermatocytes in liquid nitrogen can be used for preparing SC.