The Effects of Different Cryoprotectants on the Cryo preservation of Rabbit Embryonic Stem Cells

YUAN Xiao-hua; WANG Shu-fen; YANG Shi-hua; LI Ya-hui; CHEN Hong-wei; JI Wei-zhi <SUP>; *</SUP>

YUAN Xiao-hua, WANG Shu-fen, YANG Shi-hua, LI Ya-hui, CHEN Hong-wei, JI Wei-zhi , *. 2007. The Effects of Different Cryoprotectants on the Cryo preservation of Rabbit Embryonic Stem Cells. Zoological Research, 28(1): 81-87.

Citation:

The Effects of Different Cryoprotectants on the Cryo preservation of Rabbit Embryonic Stem Cells

Graphical Abstract

Graphical Abstract

Abstract

Abstract

An effective freezing-thawing technique is crucial for the clinical application of newly derived rabbit embryonic stem (ES) cells. The aim of this study was try to find an optimal cryopreservation protocol for rabbit embryonic stem cells using slow freezing-rapid thawing without a programmable freezer. We tested the effects of the following cryoprotective agents (CPAs) on the post-thaw survival, proliferation and differentiation capacity of rabbit embryonic stem cells: ethylene glycol (EG), dimethyl sulphoxide (Me₂SO, DMSO), trehalose and glutamine. Trypan blue exclusion tests showed that, among the CPA treatments in this study, EG was more toxic to rabbit embryonic stem cells than DMSO. The highest survival rate (83.7%) was obtained when the rabbit embryonic stem cells was cryopreserved with 10%DMSO and 20 mmol/L glutamine in the ES cell culture media. Thawed ES cells kept their pluripotency and differentiation potential.