Construction of a cDNA Library of the Prefrontal Cortex of Rhesus Monkey
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Graphical Abstract
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Abstract
Total RNA was extracted from the prefrontal cortex (PFC) of rhesus monkeys with TRIzol reagent. mRNA was further purified from total RNA using a purifying kit for mRNA. According to the manual of cDNA Synthesis Kit (200401), ZAP-cDNA Synthesis Kit (200400)，and ZAP-cDNA GigapackⅢ Gold Cloning Kit (200450) from Stratagene, we constructed a cDNA-library of the PFC of rhesus monkeys. Our aim was to identify brain-function (such as cognition and memory) related genes and gene families. The cDNA library has a capacity of 2.0×10 6 clones, and 97.3% of them are recombinant clones. The average size of the inserted cDNA fragments is 1.0 kb with the majority of inserts larger than 0.5 kb, which meets the requirement of a standard cDNA library. The PFC cDNA library constructed will be useful in cloning and sequencing brain-function related genes, and in dissecting the alternative splicing patterns of genes expressed in the brain. Meanwhile, it will also serve as a useful resource in understanding the expression regulations within the brain.
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