Possible Mechanisms Underlying Exprssion of MS3 RNA in Mouse embryonal Carcinoma Cells

The degrees of methylation of MS3 gene regions detectable with MS3 probe were found to be nearly the same in F9 genome and TDM1 genome.Nuclear transcripts of MS3 genes were detected not only in F9 cells but also in TDM1 cells.These results suggest that the failure of detection of MS3 RNA in the cytoplasm of differentiated cells may be mainly due to the defect of splicing the nuclear transcripts of MS3 genes or inability of transfer of the mature MS3 RNA from nucleus to cytoplasm.