Study on the Venom of Trimeresurus mucrosquamatus Ⅰ.Isolation of T.mucrosquamatus Venom and the Determination of its Biological and Enzymatic Activities

By means of DEAE-sephadex A-50 column chromatography,Trimeresurus mucrosquamqtus venom was separated into fourteen fractions.It contained proteolytic enzyme,5-nucleotidase,arginine esterase,etc.The enzymatic activities and biological activities of crude venom and each protein fraction were determined.The proteolytic enzyme was concentrated in the fractions 1,2,4,7,and 8.Among them the fraction 1 had the highest activity of proteolytic enzyme.Fraction 2 possessed phospholipase A activity,but not the others.Fraction 7-14 possessed arginine esterase activity.Fractions 10-14 possessed hemorrhagic principles and among them fraction 12 was the highest.Fractions 1,12-14 possessed lethal toxicity.Fractions 11-13 showed the effect of platelet aggregation.