The Establishment and Preservation of Lymphocyte Strain of Some Rare Primates

This is a report of the comparative study of the chromosomes in B95-8 cell transformed by EBV with that of the normal Sagninus oedipus studied by Hsu T.C.For the normal,the diploid chromosomes number is 2n=46 and karyotype consists of three groups:A,2 pairs submetacentric and 2 pairs of micrometacentric;B,11 pairs of submetacentric;C,7 pairs of telocentric.One large metacentric X-chromosome and a microtelocentric Y-chromosome can be differentiated easily.For the B95-8 cell which has been transformed through carrying EBV 2n=47,the A and C group of the karyotype appeared as the same as the normal Sagninus oeaipus.But for the B group,there are 3 chromosomes without pairing (one large metacentric;one large submetacentric and one microtelocentric) with the exception of No.10 submetacentric.However,the lymphocyte of #FKRhinopitheus bieti,Rhimopitheus rotellanae,Hylobates hoolock,Presbytis francoisi,Presbytis phayrei crepusculus as well as Presbytis nemaeus transformed by EBV the diploid chromosome number,G-,C-banding and Ag-NORs are all consistent with that of their normal ones.