Studies on The Mitochondrial DNA Restriction Maps and Restriction Cites Variation of Hog-Badger and Siberian Weasel

The mitochondrial DNAs (mtDNAs) from hog-badger (Arctonyx collaris) and siberian weasel (Mustela sibirica) liver were digested with 12 restriciton endonucleases,Apa I,Bgl Ⅰ,Bgl Ⅱ,Cla Ⅰ,EcoR Ⅰ,EcoR V,Hind Ⅲ,Hpa Ⅰ,Pst Ⅰ,Pvu IⅡ,Sca Ⅰ,Sal Ⅰ.The mtDNAs were also cleaved by double-enzyme digestions with the above mentioned enzymes.Restriction maps for hog-badger and siberian weasel were made according to the number and size of those fragments obtained by single and doubledigestions.Combining the previous data,the conservation and variation of mtDNA restriction sites of mammals among far ranged species were also discussed.