Morphological and Stereological Studies of The Langerhans Islet Cells in The Toad pancreas

The distribution and morphology of the A,B and D cells in toad pancreas were observed with immunohischemistry (PAP method,PAP-protein A goldsilver double staining) and electron microscopy.By PA-gold (PAG) method,the precise intracellular localization of insulin has been observed.The stereological algorthms for the conversion of the profile-size frequency distribution observed in histological section to the sphere-size frequency distribution in three-dimension space have been developed to a computer program and were applied to A,B cells in the pancreas.