Observations on New Cell line of Indian muntjac Transformed With Mnng

Lung fibroblast line of Indian muntjac (KIZ-7901) was transformed with MNNG (N-Methyl-N-nitro-N-nitrosoguanidine).The transformed Indian muntjac cells (KIZ-8401) possessed the new karyotype with chromosome number of 8 in male and polyploid.The morphology and cell cycle as well as mitotic index in MNNG-transformed muntjac cells were different from diploid cell line of Indian muntjac (KIZ-7901).Moreover,the MNNG-transformed muntjac cells possessed colony-forming capacity in vitro.