The Isolation,Purification and Some Properties of Two Toxins From the Scorpion Venom of Buthus martensii Karshi From Liaoning

The crude venom of scorpion Buthus martensii Karshi (from Liaoning) was fractionated into 12 protein peaks on CM-Sephadex C-50,Two toxins were purified from the eighth fracton by rechromatography on CM-Sephadex C-50 and gel filtration on Sehadex G-50.Their homogeneties were demonstrated by polyacrylamide gel electrophoresis,isoelectric focusing electrophoresis and SDS-polyacrylamide gel slab electrophoresis.The molecular weight of the toxins,calculated from measurement in SDS-Discontinuous polyacrylamide gel slab electrophoresis,are 8,980 and 8,660 respectively.Their isoelectric points are 7.58 and 7.90 respectivey by isoelectric focusing electrophoresis.The amion acid composition of the toxin Ⅰ was determined.The crude venom showed hyaluronidase activity but on phospholipase A2,α-amyase,arginine esterase,acetylcholinesterase,phosphomonoesterase,L-amino acid oxidase as well as proteinase activity.The LD50 of crude venom of scorpion Buthus martensii Karshi was found to be 10.3 μg/g for mice.The protein of molecular weight less than 10,000 dalton could be determined by SDS-disc polyacrylamide gel slab electrophoresis (13%).The results obtained were more satisfactory.