Identification of Mouse Lymph Node and Thymus T,B Lymphocyte

The azo dye method for acid phosphatase and "Gomori" method for 5 Nucleotidase were used to identify the T,and B lymphocytes of mouse lymph node and thymus cytochemically.By employing these methods,the estimation of ACP and 5 N activities of parallel samples from the lymph node and thymus cell suspensions were carried out in order to verify the ACP and 5N positive lymphocyte percentages obtained previously.The ACP and 5 N positive lymphocytes thus estimated for lymph node were found to be 67% and 31%,whereas those for the thymus were around 97% and 10% respectively.Above mentioned results were not only well fitted with each other but also in good accordance with those already published.